PREPARATION AND MEMBRANE FLUIDITY OF NOBLE METAL NANOPARTICLES LOADED DPPC LIPOSOMES

Abstract

Noble metal nanoparticles (silver, gold and platinum) have lately attracted considerable attentions inf the applications of food, drug and cosmetics. The most effective form of noble metal nanoparticls could permeate into the cell is reported as colloidal states, not ionic fine particles. With respect to the increased interests, it is under the necessity of the research about noble metal colloids with cell membrane-like model. Silver nanoparticles have been reported as anti-microbial property and applied to the products such as soap and toothpaste. Gold nanoparticles are used as biomedical agents, natural preservative and bactericide. And platinum nanoparticles are admitted to anticancer agents, food supplements in gums or beverage. In this study, silver, gold and platinum nanoparticles were dispersed in toluene or water as colloidal solution were respectively loaded in the dipalmitoylphosphatidylcholine (DPPC) liposome by simple preparation. The morphologies of noble metal colloids loaded liposomes investigated as spherical doughnut-like shaper by OM and HVEN. Numbers of silver, gold and platinum nanoparticles were investigated as ring-like view photograph by EDS mapping images. With the additional elemental indexing of spectrum, it is further supported that the peaks of silver, gold and platinum nanoparticles were relatively highly detected in the each liposome by fluorescent anisotropy measurement. It was controlled by the types of the each noble metal nanoparticles, the dispersed medium, and the contents of each noble metal colloids.