Thermodynamic Properties of Aqueous Two-Protein Solutions

Abstract

We establish a new perturbation model to represent thermodynamic properties of binary globular protein solutions under different conditions. A new pair potential-energy function, the generalized Lennard-Jones potential, is proposed to explain the interaction between protein and salt molecules. A temperature-dependent parameter is determined from the computer simulation data for the compressibility factor. The energy parameters are then obtained from the experimental cloud-point temperature (CPT) data for lysozyme/salt solutions and also used to describe the osmotic properties of the corresponding systems with no additional model parameters.