Phosphatidic acid produced by phospholipase D inhibits Fas-induced apoptosis via Bcl-2 upregulation in A20 murine B lymphoma cells
DC Field | Value | Language |
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dc.contributor.author | 한중수 | - |
dc.date.accessioned | 2021-08-04T08:33:39Z | - |
dc.date.available | 2021-08-04T08:33:39Z | - |
dc.date.issued | 20020201 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/78537 | - |
dc.description.abstract | Increased level of phospholipase D2 (PLD2) activity induced by PLD2 gene transfection, upregulated Bcl-2 expression and suppressed Fas-induced apoptosis at the same time. When the PLD2 activity was completely down regulated, Fas-induced apoptosis was not attenuated at all. These findings suggest that PLD2 activity suppress Fas-induced apoptosis in A20 cells. Pretreatment of phosphatidic acid (PA), the enzymatic product of PLD, in A20 cells also showed increased level of Bcl-2 expression and attenuated Fas-induced apoptosis. To determine the role of PA, we blocked the downstream signaling pathways of PA by propranolol, a phosphatidate phosphohydrolase inhibitor. Inhibition of phosphatidate phosphohydrolase resulted in the complete inhibition of Fas-mediated apoptosis and Bcl-2 upregulation. These results imply that PA produced by PLD plays a role in the increase of Bcl-2 expression and inhibition of apoptosis by Fas cross-linking. To confirm that PA itself can act as survival factor in Fas-mediated apoptosis in A20 cells, we blocked PLA2 using mepacrine, a well known PLA2 inhibitor. Interestingly, inhibition of PLA2 resulted in enhanced Fas-mediated apoptosis. This finding suggests that conversion of PA into lysophosphatidic acid (LPA) and arachidonic acid by PLA2 is necessary to show anti-apoptotic effect against Fas-induced cell death in A20 cells. | - |
dc.title | Phosphatidic acid produced by phospholipase D inhibits Fas-induced apoptosis via Bcl-2 upregulation in A20 murine B lymphoma cells | - |
dc.type | Conference | - |
dc.citation.conferenceName | Signal Transduction 2002 | - |
dc.citation.conferencePlace | Luxembourg | - |
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