Coexpression of human CYP1A2 and N-acetyltransferase 2 in Chinese hamster ovary cells results in high cytotoxicity for carcinogenic heterocyclic amines
DC Field | Value | Language |
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dc.contributor.author | 박장환 | - |
dc.date.accessioned | 2021-08-04T09:35:29Z | - |
dc.date.available | 2021-08-04T09:35:29Z | - |
dc.date.created | 2021-06-30 | - |
dc.date.issued | 2000-08-24 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/80285 | - |
dc.description.abstract | Chinese hamster ovary (CHO) cell line stably expressing human CYP450 1A2 (CYP1A2), NADPH-CYP450 reductase (CYPR) and N-acetyltransferase 2 (NAT2) was established. The expression of three proteins was determined by Western blot analyses. The introduction of NAT2 cDNA to CHO.1A2BC-RSV which had been transfected with CYP1A2 and CYPR that previously established. The cytotoxicity assay and micronucleus test of 2-aminoanthracene (2-AA), 2-amono-3,4-dimethylimidazo[4,5-f]quinoxaline (MeIQx) were approximately 1,000- and 100-fold more sensitive than CHO.1A2BC-RSV cells. There were no clear differences in sensitivity treated with 2-aminofluorene (2-AF) and 2-amino-1-metyl-6-phenylimidazo[4,5-b]pyridine (PhIP). On the basis of results obtained in this study, it is suggested that the simultaneous expression of CYP1A2, CYPR and NAT2 can be used as a good research model for in vitro metabolic activation. | - |
dc.publisher | Asian Society of Toxicology | - |
dc.title | Coexpression of human CYP1A2 and N-acetyltransferase 2 in Chinese hamster ovary cells results in high cytotoxicity for carcinogenic heterocyclic amines | - |
dc.type | Conference | - |
dc.contributor.affiliatedAuthor | 박장환 | - |
dc.identifier.bibliographicCitation | The 2nd Congress of Asian Society of Toxicology, ASIATOX II | - |
dc.relation.isPartOf | The 2nd Congress of Asian Society of Toxicology, ASIATOX II | - |
dc.citation.title | The 2nd Congress of Asian Society of Toxicology, ASIATOX II | - |
dc.citation.conferencePlace | 한국, 제주도, Hyatt Regency | - |
dc.type.rims | CONF | - |
dc.description.journalClass | 1 | - |
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