TGFβ1 suppressed matrix mineralization of osteoblasts differentiation by regulating SMURF1–C/EBPβ–DKK1 axis
DC Field | Value | Language |
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dc.contributor.author | Nam, Bora | - |
dc.contributor.author | Park, Hyosun | - |
dc.contributor.author | Lee, Young Lim | - |
dc.contributor.author | Oh, Younseo | - |
dc.contributor.author | Park, Jinsung | - |
dc.contributor.author | Kim, So Yeon | - |
dc.contributor.author | Weon, Subin | - |
dc.contributor.author | Choi, Sung Hoon | - |
dc.contributor.author | Yang, Jae-Hyuk | - |
dc.contributor.author | Jo, Sungsin | - |
dc.contributor.author | Kim, Tae-Hwan | - |
dc.date.accessioned | 2021-08-02T08:28:52Z | - |
dc.date.available | 2021-08-02T08:28:52Z | - |
dc.date.created | 2021-05-11 | - |
dc.date.issued | 2020-12 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/8192 | - |
dc.description.abstract | Transforming growth factor β1 (TGFβ1) is a major mediator in the modulation of osteoblast differentiation. However, the underlying molecular mechanism is still not fully understood. Here, we show that TGFβ1 has a dual stage-dependent role in osteoblast differentiation; TGFβ1 induced matrix maturation but inhibited matrix mineralization. We discovered the underlying mechanism of the TGFβ1 inhibitory role in mineralization using human osteoprogenitors. In particular, the matrix mineralization-related genes of osteoblasts such as osteocalcin (OCN), Dickkopf 1 (DKK1), and CCAAT/enhancer-binding protein beta (C/EBPβ) were dramatically suppressed by TGFβ1 treatment. The suppressive effects of TGFβ1 were reversed with anti-TGFβ1 treatment. Mechanically, TGFβ1 decreased protein levels of C/EBPβ without changing mRNA levels and reduced both mRNA and protein levels of DKK1. The degradation of the C/EBPβ protein by TGFβ1 was dependent on the ubiquitin–proteasome pathway. TGFβ1 degraded the C/EBPβ protein by inducing the expression of the E3 ubiquitin ligase Smad ubiquitin regulatory factor 1 (SMURF1) at the transcript level, thereby reducing the C/EBPβ-DKK1 regulatory mechanism. Collectively, our findings suggest that TGFβ1 suppressed the matrix mineralization of osteoblast differentiation by regulating the SMURF1-C/EBPβ-DKK1 axis. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.title | TGFβ1 suppressed matrix mineralization of osteoblasts differentiation by regulating SMURF1–C/EBPβ–DKK1 axis | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Choi, Sung Hoon | - |
dc.contributor.affiliatedAuthor | Yang, Jae-Hyuk | - |
dc.contributor.affiliatedAuthor | Kim, Tae-Hwan | - |
dc.identifier.doi | 10.3390/ijms21249771 | - |
dc.identifier.scopusid | 2-s2.0-85098333110 | - |
dc.identifier.wosid | 000602917200001 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.21, no.24, pp.1 - 15 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.title | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.volume | 21 | - |
dc.citation.number | 24 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 15 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.subject.keywordPlus | GROWTH-FACTOR-BETA | - |
dc.subject.keywordPlus | BINDING PROTEIN-BETA | - |
dc.subject.keywordPlus | TGF-BETA | - |
dc.subject.keywordPlus | BONE-FORMATION | - |
dc.subject.keywordPlus | C/EBP-BETA | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | OSTEOGENESIS | - |
dc.subject.keywordPlus | DEGRADATION | - |
dc.subject.keywordAuthor | osteoblast differentiation | - |
dc.subject.keywordAuthor | mineralization | - |
dc.subject.keywordAuthor | TGF beta 1 | - |
dc.subject.keywordAuthor | SMURF1 | - |
dc.subject.keywordAuthor | C/EBP beta | - |
dc.subject.keywordAuthor | DKK1 | - |
dc.identifier.url | https://www.mdpi.com/1422-0067/21/24/9771 | - |
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