Biodistribution and Pharmacokinetic Study of Gemcitabine Hydrochloride Loaded Biocompatible Iron-Based Metal Organic Framework
DC Field | Value | Language |
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dc.contributor.author | Kush, Preeti | - |
dc.contributor.author | Bajaj, Tania | - |
dc.contributor.author | Kaur, Manjot | - |
dc.contributor.author | Madan, Jitender | - |
dc.contributor.author | Jain, Upendra Kumar | - |
dc.contributor.author | Kumar, Parveen | - |
dc.contributor.author | Deep, Akash | - |
dc.contributor.author | Kim, Ki-Hyun | - |
dc.date.accessioned | 2021-08-02T08:53:12Z | - |
dc.date.available | 2021-08-02T08:53:12Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2020-08 | - |
dc.identifier.issn | 1574-1443 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/9019 | - |
dc.description.abstract | This study was designed to improve bioavailability and therapeutic efficacy of Gemcitabine (GEM) with reduced side effects using MOF MIL-100 as cargo. MIL-100 was synthesized, and characterized by microscopic and spectroscopic techniques. Impregnation approach was used for encapsulation of GEM inside the MIL-100 (i.e., MIL100-GEM). In-vitro release studies of MIL100-GEM was carried out in different media (PBS, deionized water and Tris buffer, pH = 7.4, 9.5 mM) to find out the drug release mechanism. Cytotoxicity and apoptosis assays were evaluated using MTT and fluorescence-activated cell sorting (FACS) assay in MiaPaCa-2 pancreatic cancer cell lines. Biocompatibility, pharmacokinetic and biodistribution studies of MIL100-GEM were assessed in Wistar rats. MIL100-GEM exhibited high encapsulation efficiency (78.6 +/- 0.5%) and maximum payload (23.6 +/- 1%). PXRD confirmed crystallinity of MIL-100, and did not show any effect on its structural integrity after encapsulation of GEM. In-vitro release studies revealed a biphasic release pattern in PBS buffer which followed Higuchi diffusion kinetics. In-vitro cytotoxicity studies showed low IC(50)value for MIL100-GEM (3.50 +/- 1.33 mu g/ml) compared to GEM (6.22 +/- 1.55 mu g/ml), ensuring adequate cell proliferation after 72 h. Hemolysis study showed that MIL100-GEM (14.54 +/- 1.3%) had better biocompatibility than the native GEM (30.52 +/- 1.67%). Furthermore, pharmacokinetic and biodistribution studies exhibited similar to 17-fold increased bioavailability, similar to 20-fold increased distribution half-life and similar to 15-folds elimination half-life of GEM with less accumulation of drug in the kidneys. MIL-100 MOF was synthesized and characterized to address the metabolic degradation issue of GEM. Biocompatible, MIL100-GEM demonstrated efficient drug (GEM) loading and enhanced cytotoxic activity in pancreatic cancer cell line with augmented bioavailability, providing MIL-100 a promising drug cargo. Graphic MIL-100 was synthesized using a microwave-assisted method. The anticancer drug Gemcitabine Hydrochloride (GEM) was loaded into the MIL-100 using the impregnation method. Encapsulation protected the drug from its metabolic inactivation to enhance bioavailability in target organs and reduce side effects. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER | - |
dc.title | Biodistribution and Pharmacokinetic Study of Gemcitabine Hydrochloride Loaded Biocompatible Iron-Based Metal Organic Framework | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Ki-Hyun | - |
dc.identifier.doi | 10.1007/s10904-019-01417-4 | - |
dc.identifier.scopusid | 2-s2.0-85076266162 | - |
dc.identifier.wosid | 000547945500002 | - |
dc.identifier.bibliographicCitation | JOURNAL OF INORGANIC AND ORGANOMETALLIC POLYMERS AND MATERIALS, v.30, no.8, pp.2827 - 2841 | - |
dc.relation.isPartOf | JOURNAL OF INORGANIC AND ORGANOMETALLIC POLYMERS AND MATERIALS | - |
dc.citation.title | JOURNAL OF INORGANIC AND ORGANOMETALLIC POLYMERS AND MATERIALS | - |
dc.citation.volume | 30 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 2827 | - |
dc.citation.endPage | 2841 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Polymer Science | - |
dc.relation.journalWebOfScienceCategory | Polymer Science | - |
dc.subject.keywordPlus | HYDROPHILIC ANTICANCER DRUG | - |
dc.subject.keywordPlus | TARGETED DELIVERY | - |
dc.subject.keywordPlus | NUCLEOSIDE TRANSPORTERS | - |
dc.subject.keywordPlus | CHITOSAN NANOPARTICLES | - |
dc.subject.keywordPlus | ANTITUMOR-ACTIVITY | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | LIPOSOMES | - |
dc.subject.keywordPlus | ENCAPSULATION | - |
dc.subject.keywordPlus | RELEASE | - |
dc.subject.keywordPlus | CARDIOTOXICITY | - |
dc.subject.keywordAuthor | Gemcitabine hydrochloride | - |
dc.subject.keywordAuthor | Biocompatibility | - |
dc.subject.keywordAuthor | Metal-organic frameworks | - |
dc.subject.keywordAuthor | Pancreatic cancer | - |
dc.subject.keywordAuthor | Hemolysis | - |
dc.subject.keywordAuthor | Half life | - |
dc.subject.keywordAuthor | Biodistribution | - |
dc.subject.keywordAuthor | Pharmacokinetics | - |
dc.identifier.url | https://link.springer.com/article/10.1007/s10904-019-01417-4 | - |
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