Conjugate of graphene quantum dots and glutaminase for the sensing of L-glutamine: Electrochemical vs. fluorescent sensing approaches
DC Field | Value | Language |
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dc.contributor.author | Devi, Pooja | - |
dc.contributor.author | Kukkar, Deepak | - |
dc.contributor.author | Kaur, Manpreet | - |
dc.contributor.author | Thakur, Anupma | - |
dc.contributor.author | Kim, Ki-Hyun | - |
dc.contributor.author | Kukkar, Preeti | - |
dc.contributor.author | Kaur, Kamalpreet | - |
dc.contributor.author | Kaur, Harsimran | - |
dc.date.accessioned | 2021-07-30T04:42:52Z | - |
dc.date.available | 2021-07-30T04:42:52Z | - |
dc.date.created | 2021-07-14 | - |
dc.date.issued | 2021-08 | - |
dc.identifier.issn | 1387-7003 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/950 | - |
dc.description.abstract | This research describes the synthesis of a covalent bioconjugate of graphene quantum dots (GQDs) and glutaminase (10 units·mg−1) for electrochemical and photoluminescent (PL) detection of L-glutamine (Gln). As synthesized GQDs exhibited uniform size distribution, (average diameter ~5–10 nm, and discrete blue emission characteristics (λex = 391 ± 2 nm and λem = 480 ± 2 nm)). Accordingly, the immobilization of glutaminase over the surface of the GQDs was confirmed by the characteristic Fourier-transform infrared (FTIR) spectroscopy peaks of the amide bond at 1,703 and 1,390 cm−1. The conjugate was used to detect Gln using electrochemical and PL approaches on a parallel basis over a concentration range of 100–1,000 µM. Electrochemical analysis of Gln using cyclic voltammetry revealed a limit of detection (LOD) and limit of quantification (LOQ) values of 360.4 µM and 1,100 µM, respectively. In comparison, quantification of Gln based on the PL approach yielded a Stern-Volmer quenching constant, LOD, and LOQ values of 2.2 × 103 M−1, 9.42 µM, and 28.57 µM, respectively. As such, the PL spectroscopy-based analysis was seen to have better sensitivity for Gln quantification than the electrochemical analysis. The PL-based approach is a more recommendable option for Gln quantification with high sensitivity and rapidity. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Elsevier B.V. | - |
dc.title | Conjugate of graphene quantum dots and glutaminase for the sensing of L-glutamine: Electrochemical vs. fluorescent sensing approaches | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Ki-Hyun | - |
dc.identifier.doi | 10.1016/j.inoche.2021.108745 | - |
dc.identifier.scopusid | 2-s2.0-85108403439 | - |
dc.identifier.wosid | 000672567600002 | - |
dc.identifier.bibliographicCitation | Inorganic Chemistry Communications, v.130, pp.1 - 7 | - |
dc.relation.isPartOf | Inorganic Chemistry Communications | - |
dc.citation.title | Inorganic Chemistry Communications | - |
dc.citation.volume | 130 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 7 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Inorganic & Nuclear | - |
dc.subject.keywordPlus | SENSOR | - |
dc.subject.keywordPlus | ENZYME | - |
dc.subject.keywordPlus | CARBON | - |
dc.subject.keywordPlus | OXIDE | - |
dc.subject.keywordPlus | BIOSENSOR | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | ENHANCEMENT | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | PROBE | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordAuthor | Cyclic voltammetry | - |
dc.subject.keywordAuthor | Glutaminase | - |
dc.subject.keywordAuthor | Glutamine | - |
dc.subject.keywordAuthor | Graphene quantum dots | - |
dc.subject.keywordAuthor | Sensing | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S1387700321003002?via%3Dihub | - |
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