Self-Organized Liver Microtissue on a Bio-Functional Surface: The Role of Human Adipose-Derived Stromal Cells in Hepatic Function
DC Field | Value | Language |
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dc.contributor.author | Hong, Seokheon | - |
dc.contributor.author | Oh, Seung Ja | - |
dc.contributor.author | Choi, Dongho | - |
dc.contributor.author | Hwang, Yongsung | - |
dc.contributor.author | Kim, Sang-Heon | - |
dc.date.accessioned | 2021-08-02T09:25:56Z | - |
dc.date.available | 2021-08-02T09:25:56Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2020-07 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/9679 | - |
dc.description.abstract | The maintenance of hepatocyte function is a critical research topic in liver tissue engineering. Although an increasing number of strategies have been developed, liver tissue engineering using hepatocytes as a therapeutic alternative remains challenging owing to its poor efficacy. In this study, we developed a multicellular hepatic microtissue to enhance the function of induced hepatic precursor cells. Mouse induced hepatic precursor cells (miHeps) were self-organized in 3D with human adipose-derived stem cells (hASCs) on a bio-functional matrix. We found that hepatic phenotypes, such as levels of albumin, asialoglycoprotein receptor-1, and cytochrome P450, were enhanced in miHeps-hASC microtissue comprising miHeps and hASCs relative to two-dimensional-cultured miHeps-hASCs. Additionally, the secretome of 3D-cultured hASCs increased the hepatic function of mature miHeps. Furthermore, hepatic gene expression was reduced in mature miHeps treated with conditioned media of hypoxia-inducible factor 1 alpha (HIF1 alpha)-depleted hASCs relative to that with conditioned media of control hASCs. Our results suggested that the hepatic function of 3D-co-cultured miHeps could be enhanced by HIF1 alpha-dependent factors secreted from stromal cells. This study provides an insight into the factors regulating hepatic function and shows that self-organized hepatic microtissue could act as liver spheroids for liver regenerative medicine and liver toxicity tests. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.title | Self-Organized Liver Microtissue on a Bio-Functional Surface: The Role of Human Adipose-Derived Stromal Cells in Hepatic Function | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Choi, Dongho | - |
dc.identifier.doi | 10.3390/ijms21134605 | - |
dc.identifier.scopusid | 2-s2.0-85087112007 | - |
dc.identifier.wosid | 000550265000001 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.21, no.13, pp.1 - 21 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.title | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.volume | 21 | - |
dc.citation.number | 13 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 21 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.subject.keywordPlus | MESENCHYMAL STEM-CELLS | - |
dc.subject.keywordPlus | 3-DIMENSIONAL COCULTURE | - |
dc.subject.keywordPlus | MOUSE FIBROBLASTS | - |
dc.subject.keywordPlus | TISSUE | - |
dc.subject.keywordPlus | HEPATOCYTES | - |
dc.subject.keywordPlus | MICROENVIRONMENTS | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | MAINTENANCE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | CLUSTERS | - |
dc.subject.keywordAuthor | multicellular hepatic microtissue | - |
dc.subject.keywordAuthor | human adipose stem cell | - |
dc.subject.keywordAuthor | induced hepatocyte | - |
dc.subject.keywordAuthor | hypoxia-inducible factor 1 alpha | - |
dc.subject.keywordAuthor | hepatocyte maturation | - |
dc.identifier.url | https://www.mdpi.com/1422-0067/21/13/4605 | - |
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