Whole Cell Bioconversion of Ricinoleic Acid to 12-Ketooleic Acid by Recombinant Corynebacterium glutamicum-Based Biocatalyst
DC Field | Value | Language |
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dc.contributor.author | Lee, Byeonghun | - |
dc.contributor.author | Lee, Saebom | - |
dc.contributor.author | Kim, Hyeonsoo | - |
dc.contributor.author | Jeong, Kijun | - |
dc.contributor.author | Park, Jinbyung | - |
dc.contributor.author | Park, Kyungmoon | - |
dc.contributor.author | Lee, Jinwon | - |
dc.date.available | 2020-07-10T07:21:35Z | - |
dc.date.created | 2020-07-06 | - |
dc.date.issued | 2015-04 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/10059 | - |
dc.description.abstract | The biocatalytic efficiency of recombinant Corynebacterium glutamicum ATCC 13032 expressing the secondary alcohol dehydrogenase of Micrococcus luteus NCTC2665 was studied. Recombinant C. glutamicum converts ricinoleic acid to a product, identified by gas chromatography/mass spectrometry as 12-ketooleic acid (12-oxo-cis-9-octadecenoic acid). The effects of pH, reaction temperature, and non-ionic detergent on recombinant C. glutamiucm whole cell bioconversion were examined. The determined optimal conditions for production of 12-ketooleic acid are pH 8.0, 35 degrees C, and 0.05 g/l Tween80. Under these conditions, recombinant C. glutamicum produces 3.3 mM 12-ketooleic acid, with a 72% (mol/mol) maximum conversion yield, and 1.1 g/l/h volumetric productivity in 2 h; and 3.9 mM 12-ketooleic acid, with a 74% (mol/mol) maximum conversion yield, and 0.69 g/l/h maximum volumetric productivity in 4 h of fermentation. This study constitutes the first report of significant production of 12-ketooleic acid using a recombinant Corynebacterium glutamicum-based biocatalyst. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.subject | OLEIC-ACID | - |
dc.subject | FATTY-ACIDS | - |
dc.subject | 10-KETOSTEARIC ACID | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | NRRL B-14859 | - |
dc.subject | CONVERSION | - |
dc.subject | DETERGENTS | - |
dc.subject | EXPRESSION | - |
dc.title | Whole Cell Bioconversion of Ricinoleic Acid to 12-Ketooleic Acid by Recombinant Corynebacterium glutamicum-Based Biocatalyst | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Kyungmoon | - |
dc.identifier.doi | 10.4014/jmb.1501.01001 | - |
dc.identifier.scopusid | 2-s2.0-84948688399 | - |
dc.identifier.wosid | 000353664000005 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.25, no.4, pp.452 - 458 | - |
dc.relation.isPartOf | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 25 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 452 | - |
dc.citation.endPage | 458 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001984095 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | OLEIC-ACID | - |
dc.subject.keywordPlus | FATTY-ACIDS | - |
dc.subject.keywordPlus | 10-KETOSTEARIC ACID | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | NRRL B-14859 | - |
dc.subject.keywordPlus | CONVERSION | - |
dc.subject.keywordPlus | DETERGENTS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordAuthor | 12-Ketooleic acid | - |
dc.subject.keywordAuthor | ricinoleic acid | - |
dc.subject.keywordAuthor | secondary alcohol dehydrogenase | - |
dc.subject.keywordAuthor | whole cell bioconversion | - |
dc.subject.keywordAuthor | Corynebacterium glutamicum | - |
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