Detection and destruction of HER2-positive cancer cells by Ultra Quenchbody-siRNA complex
DC Field | Value | Language |
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dc.contributor.author | Dong, Jinhua | - |
dc.contributor.author | Oka, Yuya | - |
dc.contributor.author | Jeong, Hee-Jin | - |
dc.contributor.author | Ohmuro-Matsuyama, Yuki | - |
dc.contributor.author | Ueda, Hiroshi | - |
dc.date.available | 2020-07-10T07:42:23Z | - |
dc.date.created | 2020-07-06 | - |
dc.date.issued | 2020-05 | - |
dc.identifier.issn | 0006-3592 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/10984 | - |
dc.description.abstract | Ultra Quenchbody (UQ-body) is a biosensor that utilizes the quenching behavior of the fluorescent dye linked to the antibody V region. When the corresponding antigen is bound to the UQ-body, the fluorescence is restored and allows the detection of target molecules easily and sensitively. In this paper, we constructed UQ-bodies to sensitively detect the human epidermal growth factor receptor 2 (HER2) cancer marker in solution or on cancer cells, which was further used to kill the cancer cells. A synthetic Fab fragment of anti-HER2 antibody Fab37 with many Trp residues at hypervariable region was prepared and labeled with fluorescent dyes to obtain the UQ-bodies. The UQ-body could detect HER2 in solution at concentrations as low as 20 pM with an EC50 of 0.3 nM with a fourfold response. Fluorescence imaging of HER2-positive cells was successfully performed without any washing steps. To deliver small interfering RNA (siRNA) to cancer cells, a modified UQ-body with C-terminal 9R sequence was also prepared. HER2-positive cancer cells were effectively killed by polo-like kinase 1 siRNA intracellularly delivered by the UQ-body-9R. The novel approach employing siRNA-empowered UQ-body could detect and image the HER2 antigen easily and sensitively, and effectively kill the HER2-positive cancer cells. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.title | Detection and destruction of HER2-positive cancer cells by Ultra Quenchbody-siRNA complex | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Jeong, Hee-Jin | - |
dc.identifier.doi | 10.1002/bit.27302 | - |
dc.identifier.scopusid | 2-s2.0-85079706185 | - |
dc.identifier.wosid | 000513735300001 | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY AND BIOENGINEERING, v.117, no.5, pp.1259 - 1269 | - |
dc.relation.isPartOf | BIOTECHNOLOGY AND BIOENGINEERING | - |
dc.citation.title | BIOTECHNOLOGY AND BIOENGINEERING | - |
dc.citation.volume | 117 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 1259 | - |
dc.citation.endPage | 1269 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 3 | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | ANTIBODY | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | DYES | - |
dc.subject.keywordAuthor | antibody drug conjugate | - |
dc.subject.keywordAuthor | cancer imaging | - |
dc.subject.keywordAuthor | fluorescence quenching | - |
dc.subject.keywordAuthor | high throughput screening | - |
dc.subject.keywordAuthor | polo-like kinase | - |
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