Induction of apoptosis in indole-3-carbinol-treated lung cancer H1299 cells via ROS level elevation
- Authors
- Lim; H.M.; Park, See-hyoung; S.-H.; Nam; M.J.
- Issue Date
- May-2021
- Publisher
- SAGE Publications Ltd
- Keywords
- apoptosis; H1299; I3C; lung cancer; reactive oxygen species (ROS)
- Citation
- Human and Experimental Toxicology, v.40, no.5, pp.812 - 825
- Journal Title
- Human and Experimental Toxicology
- Volume
- 40
- Number
- 5
- Start Page
- 812
- End Page
- 825
- URI
- https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/12452
- DOI
- 10.1177/0960327120969968
- ISSN
- 0960-3271
- Abstract
- This study was focused on investigating the anticancer potential of indole-3-carbinol (I3C) against lung cancer H1299 cells via an increase in ROS levels. To investigate the induction of growth arrest and/or cell death in H1299 cells, a cell cycle arrest assay, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick-end labeling (TUNEL) assay, and reactive oxygen species (ROS) detection assay were performed. Through the TUNEL assay, we detected I3C-induced DNA fragmentation. Fluorescence-activated cell sorting (FACS) analysis showed that I3C induced an increase in ROS levels and apoptotic rate in a dose- and time-dependent manner in H1299 cells. Western blotting demonstrated that activated forms of caspase-3, caspase-7, caspase-9, and poly (ADP-ribose) polymerase (PARP) were increased in I3C-treated H1299 cells following treatment with I3C. Furthermore, protein expression levels of FOXO3, bim, bax, and phosphorylated ERK and JNK were increased, while those of pAkt, Bcl-xL, and Bcl-2 were decreased by I3C treatment of H1299 cells. To confirm the relationship between cell apoptosis and ROS generation, H1299 cells were treated with I3C simultaneously with N-acetylcysteine (NAC), and it was shown that ROS levels decreased and viability increased. Moreover, in western blot analysis, expression of anti-apoptotic proteins (thioredoxin1, peroxiredoxin-1, Bcl-2, and Bcl-xL) in I3C-treated cells was evidently downregulated and pro-apoptotic proteins (active ASK1 and cleaved PARP) were upregulated compared to cells co-treated with NAC. The study showed that I3C induced downregulation of ROS regulator proteins and elevation of ROS, thus activating apoptotic signaling cascades in human lung cancer H1299 cells. © The Author(s) 2020.
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Collections - College of Science and Technology > Department of Biological and Chemical Engineering > 1. Journal Articles
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