Development of glutaric acid production consortium system with ��-ketoglutaric acid regeneration by glutamate oxidase in Escherichia coli
DC Field | Value | Language |
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dc.contributor.author | Yang | - |
dc.contributor.author | S.-Y. | - |
dc.contributor.author | Choi | - |
dc.contributor.author | T.-R. | - |
dc.contributor.author | Jung | - |
dc.contributor.author | H.-R. | - |
dc.contributor.author | Park | - |
dc.contributor.author | Y.-L. | - |
dc.contributor.author | Han | - |
dc.contributor.author | Y.-H. | - |
dc.contributor.author | Song | - |
dc.contributor.author | H.-S. | - |
dc.contributor.author | Gurav | - |
dc.contributor.author | R. | - |
dc.contributor.author | Bhatia | - |
dc.contributor.author | S.K. | - |
dc.contributor.author | Park, Kyoungmoon | - |
dc.contributor.author | K. | - |
dc.contributor.author | Ahn | - |
dc.contributor.author | J.-O. | - |
dc.contributor.author | Yang | - |
dc.contributor.author | Y.-H. | - |
dc.date.available | 2021-03-17T07:47:18Z | - |
dc.date.created | 2021-02-26 | - |
dc.date.issued | 2020 | - |
dc.identifier.issn | 0141-0229 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/12481 | - |
dc.description.abstract | Glutaric acid is a C5 dicarboxylic acid that can be used as a building block for bioplastics. Although high concentrations of glutaric acid can be produced by fermentation or bioconversion, a large amount of α-ketoglutaric acid (α-KG) is necessary to accept the amine group from 5-aminovaleric acid. To decrease the demand for α-KG, we introduced L-glutamate oxidase (GOX) from Streptomyces mobaraensis in our previous system for cofactor regeneration in combination with a glutaric acid production system from 5-aminovaleric acid. To enhance glutaric acid production, critical factors were optimized such as the expression vector, pH, temperature, and cell ratio. As a result, the demand for α-KG was decreased by more than 6-fold under optimized conditions. Additionally, the effect of catalase was also demonstrated by blocking the degradation of α-KG to succinic acid because of the hydrogen peroxide. Finally, 468.5 mM glutaric acid was produced from 800 mM 5-aminovaleric acid using only 120 mM α-KG. Moreover, this system containing davBA, gabTD-nox, and gox can be applied to produce glutaric acid from L-lysine by reusing α-KG with GOX. This improved cofactor regeneration system has a potential to apply much larger production of glutaric acid. © 2019 Elsevier Inc. | - |
dc.publisher | Elsevier Inc. | - |
dc.title | Development of glutaric acid production consortium system with ��-ketoglutaric acid regeneration by glutamate oxidase in Escherichia coli | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Kyoungmoon | - |
dc.identifier.doi | 10.1016/j.enzmictec.2019.109446 | - |
dc.identifier.scopusid | 2-s2.0-85073994199 | - |
dc.identifier.bibliographicCitation | Enzyme and Microbial Technology, v.133 | - |
dc.relation.isPartOf | Enzyme and Microbial Technology | - |
dc.citation.title | Enzyme and Microbial Technology | - |
dc.citation.volume | 133 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | Catalase | - |
dc.subject.keywordAuthor | Glutamate oxidase | - |
dc.subject.keywordAuthor | Glutaric acid | - |
dc.subject.keywordAuthor | Optimization | - |
dc.subject.keywordAuthor | α-Ketoglutaric acid | - |
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