Revealing of sugar utilization systems in Halomonas sp. YLGW01 and application for poly(3-hydroxybutyrate) production with low-cost medium and easy recovery
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Ye-Lim | - |
dc.contributor.author | Song, Hun-Suk | - |
dc.contributor.author | Choi, Tae-Rim | - |
dc.contributor.author | Lee, Sun Mi | - |
dc.contributor.author | Park, Sol Lee | - |
dc.contributor.author | Lee, Hye Soo | - |
dc.contributor.author | Kim, Hyun-Joong | - |
dc.contributor.author | Bhatia, Shashi Kant | - |
dc.contributor.author | Gurav, Ranjit | - |
dc.contributor.author | Park, Kyungmoon | - |
dc.contributor.author | Yang, Yung-Hun | - |
dc.date.accessioned | 2021-09-02T02:43:08Z | - |
dc.date.available | 2021-09-02T02:43:08Z | - |
dc.date.created | 2021-03-11 | - |
dc.date.issued | 2021-01-15 | - |
dc.identifier.issn | 0141-8130 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/15630 | - |
dc.description.abstract | Poly(3-hydroxybutyrate) (PHB) is a common polyhydroxyalkanoate (PHA) with potential as an alternative for petroleum-based plastics. Previously, we reported a new strain, Halomonas sp. YLGW01, which hyperproduces PHB with 94% yield using fructose. In this study, we examined the PHB production machinery of Halomonas sp. YLGW01 in more detail by deep-genome sequencing, which revealed a 3,453,067-bp genome with 65.1% guanine-cytosine content and 3054 genes. We found two acetyl-CoA acetyltransferases (Acetoacetyl-CoA thiolase, PhaA), one acetoacetyl-CoA reductase (PhaB), two PHB synthases (PhaC1, PhaC2), PHB depolymerase (PhaZ), and Enoyl-CoA hydratase (PhaJ) in the genome, alongwith two fructose kinases and fructose transporter systems, including the phosphotransferase system (PTS) and ATP-binding transport genes. We then examined the PHB production by Halomonas sp. YLGW01 using high-fructose corn syrup (HFCS) containing fructose, glucose, and sucrose in sea water medium, resulting in 7.95 +/- 0.11 g/L PHB (content, 67.39 +/- 0.34%). PHB was recovered from Halomonas sp. YLGW01 using different detergents; the use of Tween 20 and SDS yielded micro-sized granules with high purity. Overall, these results reveal the distribution of PHB synthetic genes and the sugar utilization system in Halomonas sp. YLGW01 and suggest a possible method for PHB recovery. (C) 2020 Elsevier B.V. All rights reserved. | - |
dc.publisher | ELSEVIER | - |
dc.title | Revealing of sugar utilization systems in Halomonas sp. YLGW01 and application for poly(3-hydroxybutyrate) production with low-cost medium and easy recovery | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Kyungmoon | - |
dc.identifier.doi | 10.1016/j.ijbiomac.2020.11.163 | - |
dc.identifier.scopusid | 2-s2.0-85096958416 | - |
dc.identifier.wosid | 000606683200015 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, v.167, pp.151 - 159 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | - |
dc.citation.title | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | - |
dc.citation.volume | 167 | - |
dc.citation.startPage | 151 | - |
dc.citation.endPage | 159 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Polymer Science | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Polymer Science | - |
dc.subject.keywordPlus | RALSTONIA-EUTROPHA | - |
dc.subject.keywordPlus | POLYHYDROXYBUTYRATE | - |
dc.subject.keywordPlus | POLY-3-HYDROXYBUTYRATE | - |
dc.subject.keywordPlus | POLYHYDROXYALKANOATES | - |
dc.subject.keywordPlus | GENES | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | DEGRADATION | - |
dc.subject.keywordPlus | HYDROLASE | - |
dc.subject.keywordAuthor | Carbon utilization | - |
dc.subject.keywordAuthor | Halomonas | - |
dc.subject.keywordAuthor | PHB microbead | - |
dc.subject.keywordAuthor | Poly(3-hydroxybutyrate) | - |
dc.subject.keywordAuthor | Whole genome sequencing | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
94, Wausan-ro, Mapo-gu, Seoul, 04066, Korea02-320-1314
COPYRIGHT 2020 HONGIK UNIVERSITY. ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.