Application of l-glutamate oxidase from Streptomyces sp. X119-6 with catalase (KatE) to whole-cell systems for glutaric acid production in Escherichia coli
DC Field | Value | Language |
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dc.contributor.author | Ham, Sion | - |
dc.contributor.author | Han, Yeong-Hoon | - |
dc.contributor.author | Kim, Sang Hyun | - |
dc.contributor.author | Suh, Min Ju | - |
dc.contributor.author | Cho, Jang Yeon | - |
dc.contributor.author | Lee, Hong-Ju | - |
dc.contributor.author | Park, See-Hyoung | - |
dc.contributor.author | Park, Kyungmoon | - |
dc.contributor.author | Ahn, Jung-Oh | - |
dc.contributor.author | Joo, Jeong Chan | - |
dc.contributor.author | Bhatia, Shashi Kant | - |
dc.contributor.author | Yang, Yung-Hun | - |
dc.date.accessioned | 2021-10-12T08:43:12Z | - |
dc.date.available | 2021-10-12T08:43:12Z | - |
dc.date.created | 2021-09-30 | - |
dc.date.issued | 2021-10 | - |
dc.identifier.issn | 0256-1115 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/16342 | - |
dc.description.abstract | Whole-cell systems offer many benefits for biochemical production, such as relatively easy enzyme control and higher tolerance toward harsh environments, than purified enzymes. These systems can be applied to many bioconversion reactions, but they sometimes require cofactor regeneration units to support reactions at high substrate concentrations. Here, we examined l-glutamate oxidase (GOX) from Streptomyces sp. X119-6, which produces alpha-ketoglutarate (alpha-KG) from l-glutamate, and catalase (KatE) from Escherichia coli, which removes hydrogen peroxide generated by GOX. After optimizing the expression vector, pH, strains, culture conditions, and isopropyl beta-D-1-thiogalactopyranoside concentration, we compared their efficiency to that of a previously reported GOX from Streptomyces mobaraensis. Our results indicated that GOX from Streptomyces sp. X119-6 and KatE increased alpha-KG production by 2.76-fold. This GOX required high levels of alpha-KG as an amino donor to convert 5-aminovaleric acid to glutaric acid. Performing the reaction at pH 8 enabled us to avoid the exogenous addition of catalase, but severe substrate inhibition was observed, resulting in the production of 287 mM glutaric acid. This alpha-KG regeneration system has potential for improving production in various aminotransferase systems. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | KOREAN INSTITUTE CHEMICAL ENGINEERS | - |
dc.subject | ALPHA-KETOGLUTARIC ACID | - |
dc.subject | 5-AMINOVALERIC ACID | - |
dc.title | Application of l-glutamate oxidase from Streptomyces sp. X119-6 with catalase (KatE) to whole-cell systems for glutaric acid production in Escherichia coli | - |
dc.title.alternative | Application of L-glutamate oxidase from Streptomyces sp. X119-6 with catalase (KatE) to whole-cell systems for glutaric acid production in Escherichia coli | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, See-Hyoung | - |
dc.contributor.affiliatedAuthor | Park, Kyungmoon | - |
dc.identifier.doi | 10.1007/s11814-021-0855-8 | - |
dc.identifier.scopusid | 2-s2.0-85112734361 | - |
dc.identifier.wosid | 000685906400003 | - |
dc.identifier.bibliographicCitation | KOREAN JOURNAL OF CHEMICAL ENGINEERING, v.38, no.10, pp.2106 - 2112 | - |
dc.relation.isPartOf | KOREAN JOURNAL OF CHEMICAL ENGINEERING | - |
dc.citation.title | KOREAN JOURNAL OF CHEMICAL ENGINEERING | - |
dc.citation.volume | 38 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 2106 | - |
dc.citation.endPage | 2112 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002758004 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Engineering, Chemical | - |
dc.subject.keywordPlus | ALPHA-KETOGLUTARIC ACID | - |
dc.subject.keywordPlus | 5-AMINOVALERIC ACID | - |
dc.subject.keywordAuthor | Glutaric Acid | - |
dc.subject.keywordAuthor | alpha-Ketoglutarate | - |
dc.subject.keywordAuthor | Glutamate Oxidase | - |
dc.subject.keywordAuthor | Catalase | - |
dc.subject.keywordAuthor | Optimization | - |
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