Adipose-Derived Stem Cells and BMP-2 Delivery in Chitosan-Based 3D Constructs to Enhance Bone Regeneration in a Rat Mandibular Defect Model
DC Field | Value | Language |
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dc.contributor.author | Fan, Jiabing | - |
dc.contributor.author | Park, Hyejin | - |
dc.contributor.author | Lee, Matthew K. | - |
dc.contributor.author | Bezouglaia, Olga | - |
dc.contributor.author | Fartash, Armita | - |
dc.contributor.author | Kim, Jinku | - |
dc.contributor.author | Aghaloo, Tara | - |
dc.contributor.author | Lee, Min | - |
dc.date.accessioned | 2021-11-11T02:43:27Z | - |
dc.date.available | 2021-11-11T02:43:27Z | - |
dc.date.created | 2021-10-25 | - |
dc.date.issued | 2014-08 | - |
dc.identifier.issn | 1937-3341 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/16642 | - |
dc.description.abstract | Reconstructing segmental mandiblular defects remains a challenge in the clinic. Tissue engineering strategies provide an alternative option to resolve this problem. The objective of the present study was to determine the effects of adipose-derived stem cells (ASCs) and bone morphogenetic proteins-2 (BMP-2) in three-dimensional (3D) scaffolds on mandibular repair in a small animal model. Noggin expression levels in ASCs were downregulated by a lentiviral short hairpin RNA strategy to enhance ASC osteogenesis (ASCs(Nog-)). Chitosan (CH) and chondroitin sulfate (CS), natural polysaccharides, were fabricated into 3D porous scaffolds, which were further modified with apatite coatings for enhanced cellular responses and efficient delivery of BMP-2. The efficacy of 3D apatite-coated CH/CS scaffolds supplemented with ASCs(Nog-) and BMP-2 were evaluated in a rat critical-sized mandibular defect model. After 8 weeks postimplantation, the scaffolds treated with ASCs(Nog-) and BMP-2 significantly promoted rat mandibular regeneration as demonstrated by micro-computerized tomography, histology, and immunohistochemistry, compared with the groups treated with ASCs(Nog-) or BMP-2 alone. These results suggest that our combinatorial strategy of ASCs(Nog-) + BMP-2 in 3D apatite microenvironments can significantly promote mandibular regeneration, and these may provide a potential tissue engineering approach to repair large bony defects. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MARY ANN LIEBERT, INC | - |
dc.subject | CONTROLLED-RELEASE CARRIER | - |
dc.subject | IN-VITRO | - |
dc.subject | MORPHOGENETIC PROTEINS | - |
dc.subject | BIOMIMETIC APATITE | - |
dc.subject | COMPOSITE SCAFFOLDS | - |
dc.subject | CALVARIAL DEFECTS | - |
dc.subject | FEMORAL DEFECTS | - |
dc.subject | STROMAL CELLS | - |
dc.subject | TISSUE | - |
dc.subject | HYDROXYAPATITE | - |
dc.title | Adipose-Derived Stem Cells and BMP-2 Delivery in Chitosan-Based 3D Constructs to Enhance Bone Regeneration in a Rat Mandibular Defect Model | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Jinku | - |
dc.identifier.doi | 10.1089/ten.tea.2013.0523 | - |
dc.identifier.scopusid | 2-s2.0-84904993990 | - |
dc.identifier.wosid | 000340846500013 | - |
dc.identifier.bibliographicCitation | TISSUE ENGINEERING PART A, v.20, no.15-16, pp.2169 - 2179 | - |
dc.relation.isPartOf | TISSUE ENGINEERING PART A | - |
dc.citation.title | TISSUE ENGINEERING PART A | - |
dc.citation.volume | 20 | - |
dc.citation.number | 15-16 | - |
dc.citation.startPage | 2169 | - |
dc.citation.endPage | 2179 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.subject.keywordPlus | CONTROLLED-RELEASE CARRIER | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | MORPHOGENETIC PROTEINS | - |
dc.subject.keywordPlus | BIOMIMETIC APATITE | - |
dc.subject.keywordPlus | COMPOSITE SCAFFOLDS | - |
dc.subject.keywordPlus | CALVARIAL DEFECTS | - |
dc.subject.keywordPlus | FEMORAL DEFECTS | - |
dc.subject.keywordPlus | STROMAL CELLS | - |
dc.subject.keywordPlus | TISSUE | - |
dc.subject.keywordPlus | HYDROXYAPATITE | - |
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