Production of 2,3-butanediol by a low-acid producing Klebsiella oxytoca NBRF4
DC Field | Value | Language |
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dc.contributor.author | Han, Sung-Hyuk | - |
dc.contributor.author | Lee, Jung-Eun | - |
dc.contributor.author | Park, Kyungmoon | - |
dc.contributor.author | Park, Yong-Cheol | - |
dc.date.accessioned | 2021-11-11T04:42:32Z | - |
dc.date.available | 2021-11-11T04:42:32Z | - |
dc.date.created | 2021-11-10 | - |
dc.date.issued | 2013-01-25 | - |
dc.identifier.issn | 1871-6784 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/17198 | - |
dc.description.abstract | 2,3-Butanediol (2,3-BDO) is a value-added chemical with great potential for the industrial production of synthetic rubber, plastic and solvent. For microbial production of 2,3-BDO, in this study, Klebsiella oxytoca NBRF4 was constructed by chemical mutation and screening against NaBr, NaBrO3 and fluoroacetate. Among metabolic enzymes involved in the production of lactate, acetate and 2,3-BDO, K. oxytoca NBRF4 possessed 1.2 times lower specific activities of lactate dehydrogenase and phosphotransacetylase, and 22% higher specific acetoin reductase activity than the K. oxytoca ATCC43863 control strain. A series of batch fermentations in a defined medium and application of a statistical tool of response surface method led to the determination of optimal culture conditions: 10% dissolved oxygen level, pH 4.3 and 38 degrees C. The actual results of batch fermentation at the optimal conditions using 44 g/L glucose were coincident with the predetermined values: 14.4 g/L 2,3-BDO concentration, 0.32 g/g yield. To increase 2,3-BDO titer, fed-batch fermentation of K. oxytoca NBRF4 was performed by an intermittent feeding of 800 g/L glucose to control its concentration around 5-20 g/L in the culture broth. Finally, 34.2 g/L 2,3-BDO concentration and 0.35 g/g yield were obtained without organic acid production in 70 hours of the fed-batch culture, which were 2.4 and 1.2 times higher than those of the batch fermentation using 44 g/L glucose. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | ACETATE KINASE | - |
dc.subject | PHOSPHOTRANSACETYLASE | - |
dc.subject | BIOSYNTHESIS | - |
dc.title | Production of 2,3-butanediol by a low-acid producing Klebsiella oxytoca NBRF4 | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Kyungmoon | - |
dc.identifier.doi | 10.1016/j.nbt.2012.09.004 | - |
dc.identifier.scopusid | 2-s2.0-84872370882 | - |
dc.identifier.wosid | 000313786400010 | - |
dc.identifier.bibliographicCitation | NEW BIOTECHNOLOGY, v.30, no.2, pp.166 - 172 | - |
dc.relation.isPartOf | NEW BIOTECHNOLOGY | - |
dc.citation.title | NEW BIOTECHNOLOGY | - |
dc.citation.volume | 30 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 166 | - |
dc.citation.endPage | 172 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | ACETATE KINASE | - |
dc.subject.keywordPlus | PHOSPHOTRANSACETYLASE | - |
dc.subject.keywordPlus | BIOSYNTHESIS | - |
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