Development of a fluvoxamine detection system using a Quenchbody, a novel fluorescent biosensor
DC Field | Value | Language |
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dc.contributor.author | Sasao, Ako | - |
dc.contributor.author | Takaki, Michiyo | - |
dc.contributor.author | Jeong, Hee-Jin | - |
dc.contributor.author | Yonemitsu, Kosei | - |
dc.contributor.author | Ohtsu, Yuki | - |
dc.contributor.author | Tsutsumi, Hiroshi | - |
dc.contributor.author | Furukawa, Shota | - |
dc.contributor.author | Morioka, Hiroshi | - |
dc.contributor.author | Ueda, Hiroshi | - |
dc.contributor.author | Nishitani, Yoko | - |
dc.date.available | 2020-07-10T03:37:27Z | - |
dc.date.created | 2020-07-06 | - |
dc.date.issued | 2019-04 | - |
dc.identifier.issn | 1942-7603 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/1806 | - |
dc.description.abstract | The misuse of psychotropic drugs intended for medical treatment represents a recent worldwide public health concern. Quenchbody (Q-body) is a novel fluoroimmunosensor that can detect an antigen immediately without additional reagents or washing steps. Here, we describe creating Q-bodies for the detection of the antidepressant fluvoxamine (FLV) and determining optimal conditions to achieve the highest fluorescence intensity (FI). We prepared five Q-bodies with the fluorophore labeled at either the N- or C- terminus and with different linker lengths. Fluorescence was measurable within minutes, indicating the interaction of Q-bodies with FLV. The normalized FI (FI ratio) of the N-terminus labeled Q-body increased approximately 1.5-fold upon FLV addition; Q-bodies labeled at the C-terminus did not significantly increase FI. Among the fluorescence dyes used in this study, Rhodamine 6G labeled Q-body showed the best FI ratio. EC50 values of the N-terminus labeled Q-bodies were similar (23.2-224nM) regardless of linker length or labeling dye. We examined whether the Q-body could be applicable to serum matrix instead of phosphate-buffered saline. The intact serum interfered strongly with the Q-body fluorescence. However, the FI ratios of the Q-body for FLV-spiked serum filtrate, for which proteins were removed by filtration, showed a dose-dependency for detecting FLV levels. Deproteinization, which does not interfere with Q-body fluorescence measurements, is likely necessary to detect serum FLV with high sensitivity. This study demonstrates the potential of Q-body probes as a tool towards developing creative immunoassay applications. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | ANTIBODY | - |
dc.subject | FRAGMENT | - |
dc.subject | IMMUNOASSAY | - |
dc.subject | PROTEINS | - |
dc.subject | OVERDOSE | - |
dc.title | Development of a fluvoxamine detection system using a Quenchbody, a novel fluorescent biosensor | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Jeong, Hee-Jin | - |
dc.identifier.doi | 10.1002/dta.2520 | - |
dc.identifier.scopusid | 2-s2.0-85057277556 | - |
dc.identifier.wosid | 000465003600006 | - |
dc.identifier.bibliographicCitation | DRUG TESTING AND ANALYSIS, v.11, no.4, pp.601 - 609 | - |
dc.relation.isPartOf | DRUG TESTING AND ANALYSIS | - |
dc.citation.title | DRUG TESTING AND ANALYSIS | - |
dc.citation.volume | 11 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 601 | - |
dc.citation.endPage | 609 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | ANTIBODY | - |
dc.subject.keywordPlus | FRAGMENT | - |
dc.subject.keywordPlus | IMMUNOASSAY | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | OVERDOSE | - |
dc.subject.keywordAuthor | drug screening | - |
dc.subject.keywordAuthor | fluorescent biosensor | - |
dc.subject.keywordAuthor | fluvoxamine | - |
dc.subject.keywordAuthor | immunoassay | - |
dc.subject.keywordAuthor | recombinant antibody | - |
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