Tomentosin Displays Anti-Carcinogenic Effect in Human Osteosarcoma MG-63 Cells via the Induction of Intracellular Reactive Oxygen Species
DC Field | Value | Language |
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dc.contributor.author | Lee, Chang Min | - |
dc.contributor.author | Lee, Jongsung | - |
dc.contributor.author | Nam, Myeong Jin | - |
dc.contributor.author | Choi, Youn Soo | - |
dc.contributor.author | Park, See-Hyoung | - |
dc.date.available | 2020-07-10T03:37:36Z | - |
dc.date.created | 2020-07-06 | - |
dc.date.issued | 2019-03-21 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/1832 | - |
dc.description.abstract | Tomentosin is a natural sesquiterpene lactone extracted from various plants and is widely used as a medicine because it exhibits essential therapeutic properties. In this study, we investigated the anti-carcinogenic effects of tomentosin in human osteosarcoma MG-63 cells by performing cell migration/viability/proliferation, apoptosis, and reactive oxygen species (ROS) analysis assays. MG-63 cells were treated with various doses of tomentosin. After treatment with tomentosin, MG-63 cells were analyzed using the MTT assay, colony formation assay, cell counting assay, wound healing assay, Boyden chamber assay, zymography assay, cell cycle analysis, FITC Annexin V apoptosis assay, terminal deoxynucleotidyl transferase dUTP nick end labeling assay, western blot analysis, and ROS detection analysis. Our results indicated that tomentosin decreased cell viability and migration ability in MG-63 cells. Moreover, tomentosin induced apoptosis, cell cycle arrest, DNA damage, and ROS production in MG-63 cells. Furthermore, tomentosin-induced intracellular ROS decreased cell viability and induced apoptosis, cell cycle arrest, and DNA damage in MG-63 cells. Taken together, our results suggested that tomentosin exerted anti-carcinogenic effects in MG-63 cells by induction of intracellular ROS. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.subject | ENDOPLASMIC-RETICULUM STRESS | - |
dc.subject | PI3K/AKT PATHWAY | - |
dc.subject | PEROXIREDOXIN 1 | - |
dc.subject | CYCLE ARREST | - |
dc.subject | G2/M ARREST | - |
dc.subject | APOPTOSIS | - |
dc.subject | ROS | - |
dc.subject | CARCINOMA | - |
dc.subject | ACTIVATION | - |
dc.subject | MECHANISMS | - |
dc.title | Tomentosin Displays Anti-Carcinogenic Effect in Human Osteosarcoma MG-63 Cells via the Induction of Intracellular Reactive Oxygen Species | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, See-Hyoung | - |
dc.identifier.doi | 10.3390/ijms20061508 | - |
dc.identifier.scopusid | 2-s2.0-85063945872 | - |
dc.identifier.wosid | 000465523400120 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.20, no.6 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.title | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.volume | 20 | - |
dc.citation.number | 6 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.subject.keywordPlus | ENDOPLASMIC-RETICULUM STRESS | - |
dc.subject.keywordPlus | PI3K/AKT PATHWAY | - |
dc.subject.keywordPlus | PEROXIREDOXIN 1 | - |
dc.subject.keywordPlus | CYCLE ARREST | - |
dc.subject.keywordPlus | G2/M ARREST | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | ROS | - |
dc.subject.keywordPlus | CARCINOMA | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordAuthor | osteosarcoma | - |
dc.subject.keywordAuthor | tomentosin | - |
dc.subject.keywordAuthor | reactive oxygen species (ROS) | - |
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