Identification of Factors Regulating Escherichia coli 2,3-Butanediol Production by Continuous Culture and Metabolic Flux Analysis
- Authors
- Lu, Mingshou; Lee, Soojin; Kim, Borim; Park, Changhun; Oh, Minkyu; Park, Kyungmoon; Lee, Sang Yup; Lee, Jinwon
- Issue Date
- May-2012
- Publisher
- KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
- Keywords
- 2,3-Butanediol fermentation; continuous culture; pH influence; metabolic flux analysis; intracellular metabolic measurements
- Citation
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.22, no.5, pp.659 - 667
- Journal Title
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
- Volume
- 22
- Number
- 5
- Start Page
- 659
- End Page
- 667
- URI
- https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/18963
- DOI
- 10.4014/jmb.1112.12018
- ISSN
- 1017-7825
- Abstract
- 2,3-Butanediol (2,3-BDO) is an organic compound with a wide range of industrial applications. Although Escherichia call is often used for the production of organic compounds, the wild-type E. coli does not contain two essential genes in the 2,3-BDO biosynthesis pathway, and cannot ferment 2,3-BDO. Therefore, a 2,3-BDO biosynthesis mutant strain of Escherichia coli was constructed and cultured. To determine the optimum culture factors for 2,3-BDO production, experiments were conducted under different culture environments ranging from strongly acidic to neutral pH. The extracellular metabolite profiles were obtained using high-performance liquid chromatography (HPLC), and the intracellular metabolite profiles were analyzed by ultra-performance liquid chromatography and quadruple time-of-flight mass spectrometry (UPLC/Q-TOF-MS). Metabolic flux analysis (MFA) was used to integrate these profiles. The metabolite profiles showed that 2,3-BDO production favors an acidic environment (pH 5), whereas cell mass favors a neutral environment. Furthermore, when the pH of the culture fell below 5, both the cell growth and 2,3-BDO production were inhibited.
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