A simple and cost-effective method to prepare template DNAs for cell-free protein synthesis
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Byun, Ju-Young | - |
dc.contributor.author | Park, Kyungmoon | - |
dc.contributor.author | Kim, Dong-Myung | - |
dc.date.accessioned | 2021-12-17T01:44:30Z | - |
dc.date.available | 2021-12-17T01:44:30Z | - |
dc.date.created | 2021-12-16 | - |
dc.date.issued | 2010-01 | - |
dc.identifier.issn | 0256-1115 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/20859 | - |
dc.description.abstract | The scale-up of cell-free protein synthesis reactions involves the preparation of large amounts of template DNA. While ion-exchange column chromatography methods have commonly been used to obtain purified plasmid DNA for cell-free protein synthesis reactions, these methods are costly and difficult to expand to a large scale. In this work, we report that the routine isopropyl alcohol (IPA) precipitation method can be used to prepare cell-free-expressible DNA when the co-precipitated proteins are removed. Compared to column-purification procedures, the IPA-precipitation offers obvious advantages with respect to the cost and scaling-up of template preparation, and we believe that our finding will contribute to making cell-free protein synthesis system more practical for the rapid production of preparative amounts of recombinant proteins. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | KOREAN INSTITUTE CHEMICAL ENGINEERS | - |
dc.subject | GEL-ELECTROPHORESIS | - |
dc.subject | SYNTHESIS SYSTEM | - |
dc.subject | EXTRACTS | - |
dc.title | A simple and cost-effective method to prepare template DNAs for cell-free protein synthesis | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Kyungmoon | - |
dc.identifier.doi | 10.1007/s11814-009-0294-4 | - |
dc.identifier.scopusid | 2-s2.0-74249089411 | - |
dc.identifier.wosid | 000273684300028 | - |
dc.identifier.bibliographicCitation | KOREAN JOURNAL OF CHEMICAL ENGINEERING, v.27, no.1, pp.179 - 182 | - |
dc.relation.isPartOf | KOREAN JOURNAL OF CHEMICAL ENGINEERING | - |
dc.citation.title | KOREAN JOURNAL OF CHEMICAL ENGINEERING | - |
dc.citation.volume | 27 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 179 | - |
dc.citation.endPage | 182 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001399050 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Engineering, Chemical | - |
dc.subject.keywordPlus | GEL-ELECTROPHORESIS | - |
dc.subject.keywordPlus | SYNTHESIS SYSTEM | - |
dc.subject.keywordPlus | EXTRACTS | - |
dc.subject.keywordAuthor | Plasmid | - |
dc.subject.keywordAuthor | Cell-free Protein Synthesis | - |
dc.subject.keywordAuthor | Scale-up | - |
dc.subject.keywordAuthor | Extraction | - |
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