Immobilization of enzymes within hydrogel microparticles to create optical biosensors for the detection of organophosphorus compounds
- Authors
- Kim, Bumsang; Lee, Youngsik; Lee, Kangtaek; Koh, Won-Gun
- Issue Date
- Jul-2009
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Enzyme immobilization; Enzyme activity; Optical enzyme-biosensors; Hydrogel microparticles; Enzyme-fluorophore conjugates; Organophosphorus compounds
- Citation
- CURRENT APPLIED PHYSICS, v.9, no.4, pp.E225 - E228
- Journal Title
- CURRENT APPLIED PHYSICS
- Volume
- 9
- Number
- 4
- Start Page
- E225
- End Page
- E228
- URI
- https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/21834
- DOI
- 10.1016/j.cap.2009.06.043
- ISSN
- 1567-1739
- Abstract
- As a first step in the development of an optical biosensor for the detection of organophosphorus compounds, we describe the immobilization of conjugates of enzyme (AChE) and pH-sensitive fluorophore (SNAFL-1) within the PEG hydrogel microparticles via a dispersion photopolymerization. The fluorescent response of the PEG microparticles containing AChE-SNAFL conjugates showed that AChE-SNAFL conjugates were successfully encapsulated within the microparticles and that AChE was still active after the encapsulation procedure. Using a quantitative analysis of enzyme activity, we found that 70% of AChE activity was maintained after conjugation and encapsulation. A leaching test showed that there was no significant AChE leaching into the surrounding media. These results indicate that AChE-SNAFL conjugates could be encapsulated within PEG hydrogel microparticles without a significant loss of the enzyme activity and that these microparticles could potentially be used as optical biosensors for the detection of organophosphorus compounds. (C) 2009 Elsevier B.V. All rights reserved.
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