Effect of Glutaraldehyde Treatment on Stability of Permeabilized Ochrobactrum anthropi SY509 in Nitrate Removal
DC Field | Value | Language |
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dc.contributor.author | Park, Young Tae | - |
dc.contributor.author | Park, Jae Yeon | - |
dc.contributor.author | Park, Kyungmoon | - |
dc.contributor.author | Choi, Suk Soon | - |
dc.contributor.author | Yoo, Young Je | - |
dc.date.accessioned | 2022-01-13T06:41:46Z | - |
dc.date.available | 2022-01-13T06:41:46Z | - |
dc.date.created | 2022-01-04 | - |
dc.date.issued | 2008-11 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/22644 | - |
dc.description.abstract | For practical application, the stability of permeabilized Ochrobactrum anthropi SY509 needs to be increased, as its half-life of enzymatic denitrification is only 90 days. As the cells become viable after permeabilization treatment, this can cause decreased activity in a long-term operation and induce breakage of the immobilization matrix. However, the organic solvent concentration causing zero cell viability was 50%, which is too high for industrial application. Thus, whole-cell immobilization using glutaraldehyde was performed, and 0.1% (v/v) glutaraldehyde was determined as the optimum concentration to maintain activity and increase the half-life. It was also found that 0.1% (v/v) glutaraldehyde reacted with 41.9% of the total amine residues on the surface of the cells during the treatment. As a result, the half-life of the permeabilized cells was increased from 90 to 210 days by glutaraldehyde treatment after permeabilization, and no cell viability was detected. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.subject | ESCHERICHIA-COLI-CELLS | - |
dc.subject | BETA-GALACTOSIDASE | - |
dc.subject | SURFACE | - |
dc.subject | IMMOBILIZATION | - |
dc.subject | STABILIZATION | - |
dc.subject | REDUCTION | - |
dc.subject | PROTEIN | - |
dc.subject | ENZYME | - |
dc.title | Effect of Glutaraldehyde Treatment on Stability of Permeabilized Ochrobactrum anthropi SY509 in Nitrate Removal | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Kyungmoon | - |
dc.identifier.doi | 10.4014/jmb.0800.094 | - |
dc.identifier.scopusid | 2-s2.0-64549161607 | - |
dc.identifier.wosid | 000261237000011 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.18, no.11, pp.1803 - 1808 | - |
dc.relation.isPartOf | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 18 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 1803 | - |
dc.citation.endPage | 1808 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001293146 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI-CELLS | - |
dc.subject.keywordPlus | BETA-GALACTOSIDASE | - |
dc.subject.keywordPlus | SURFACE | - |
dc.subject.keywordPlus | IMMOBILIZATION | - |
dc.subject.keywordPlus | STABILIZATION | - |
dc.subject.keywordPlus | REDUCTION | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | ENZYME | - |
dc.subject.keywordAuthor | Denitrification | - |
dc.subject.keywordAuthor | permeabilization | - |
dc.subject.keywordAuthor | glutaraldehyde | - |
dc.subject.keywordAuthor | stability | - |
dc.subject.keywordAuthor | immobilization | - |
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