Enhanced production of epsilon-caprolactone by overexpression of NADPH-regenerating glucose 6-phosphate dehydrogenase in recombinant Escherichia coli harboring cyclohexanone monooxygenase gene
DC Field | Value | Language |
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dc.contributor.author | Lee, Won-Heong | - |
dc.contributor.author | Park, Jin-Byung | - |
dc.contributor.author | Park, Kyungmoon | - |
dc.contributor.author | Kim, Myoung-Dong | - |
dc.contributor.author | Seo, Jin-Ho | - |
dc.date.accessioned | 2022-01-14T07:42:10Z | - |
dc.date.available | 2022-01-14T07:42:10Z | - |
dc.date.created | 2022-01-14 | - |
dc.date.issued | 2007-08 | - |
dc.identifier.issn | 0175-7598 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/23559 | - |
dc.description.abstract | Whole-cell conversion of cyclohexanone to epsilon- was attempted by recombinant Escherichia coli BL21(DE3) expressing cyclohexanone monooxygenase (CHMO) of Acinetobacter calcoaceticus NCIMB 9871. High concentrations of cyclohexanone and epsilon-caprolactone reduced CHMO-mediated bioconversion of cyclohexanone to epsilon-caprolactone in the resting recombinant E. coli cells. Metabolically active cells were employed by adopting a fed-batch culture to improve the production of epsilon-caprolactone from cyclohexanone. A glucose-limited fed-batch Baeyer-Villiger oxidation where a cyclohexanone level was maintained less than 6 g/l resulted in a maximum epsilon-caprolactone concentration of 11.0 g/l. The maximum epsilon-caprolactone concentration was improved further to 15.3 g/l by coexpression of glucose-6-phosphate dehydrogenase, an NADPH-generating enzyme encoded by the zwf gene which corresponded to a 39% enhancement in epsilon-caprolactone concentration compared with the control experiment performed under the same conditions. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER | - |
dc.subject | BAEYER-VILLIGER OXIDATION | - |
dc.subject | PILOT-SCALE PRODUCTION | - |
dc.subject | BIOCATALYST | - |
dc.subject | OXYGEN | - |
dc.subject | ZWF | - |
dc.subject | GND | - |
dc.title | Enhanced production of epsilon-caprolactone by overexpression of NADPH-regenerating glucose 6-phosphate dehydrogenase in recombinant Escherichia coli harboring cyclohexanone monooxygenase gene | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Kyungmoon | - |
dc.identifier.doi | 10.1007/s00253-007-1016-7 | - |
dc.identifier.scopusid | 2-s2.0-34547114478 | - |
dc.identifier.wosid | 000248299800009 | - |
dc.identifier.bibliographicCitation | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.76, no.2, pp.329 - 338 | - |
dc.relation.isPartOf | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.title | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 76 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 329 | - |
dc.citation.endPage | 338 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | BAEYER-VILLIGER OXIDATION | - |
dc.subject.keywordPlus | PILOT-SCALE PRODUCTION | - |
dc.subject.keywordPlus | BIOCATALYST | - |
dc.subject.keywordPlus | OXYGEN | - |
dc.subject.keywordPlus | ZWF | - |
dc.subject.keywordPlus | GND | - |
dc.subject.keywordAuthor | cyclohexanone monooxygenase | - |
dc.subject.keywordAuthor | Escherichia coli | - |
dc.subject.keywordAuthor | glucose 6-phosphate dehydrogenase | - |
dc.subject.keywordAuthor | fed-batch | - |
dc.subject.keywordAuthor | substrate/product inhibition | - |
dc.subject.keywordAuthor | NADPH | - |
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