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Preparation method for Escherichia coliS30 extracts completely dependent upon tRNA addition to catalyze cell-free protein synthesis

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dc.contributor.authorAhn, Jin-Ho-
dc.contributor.authorHwang, Mi-Yeon-
dc.contributor.authorOh, In-Seok-
dc.contributor.authorPark, Kyung-Moon-
dc.contributor.authorHahn, Geun-Hee-
dc.contributor.authorCho, Cha-Yong-
dc.contributor.authorKim, Dong-Myung-
dc.date.accessioned2022-02-07T05:41:24Z-
dc.date.available2022-02-07T05:41:24Z-
dc.date.created2022-02-07-
dc.date.issued2006-09-
dc.identifier.issn1226-8372-
dc.identifier.urihttps://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/24515-
dc.description.abstractA simple method for depleting E. coli S30 extracts of endogenous tRNA has been developed. An ethanolamine-Sepharose (R) column equilibrated with water selectively captured the tRNA molecules in E. coli S30 extracts. As a result, S30 extracts filtered through this column became completely dependent upon the addition of exogenous tRNA to mediate cell-free protein synthesis reactions. We anticipate that the procedures developed and described will be particularly useful for in vitro suppression reaction studies designed to introduce unnatural amino acids into protein molecules.-
dc.language영어-
dc.language.isoen-
dc.publisherKOREAN SOC BIOTECHNOLOGY & BIOENGINEERING-
dc.subjectSITE-SPECIFIC INCORPORATION-
dc.subjectNONNATURAL AMINO-ACIDS-
dc.subjectGENETIC-CODE-
dc.subjectSYNTHESIS SYSTEM-
dc.subjectCOLI-
dc.subjectCODONS-
dc.subjectPROBES-
dc.titlePreparation method for Escherichia coliS30 extracts completely dependent upon tRNA addition to catalyze cell-free protein synthesis-
dc.typeArticle-
dc.contributor.affiliatedAuthorPark, Kyung-Moon-
dc.identifier.doi10.1007/BF02932309-
dc.identifier.scopusid2-s2.0-33750688836-
dc.identifier.wosid000241824400008-
dc.identifier.bibliographicCitationBIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.11, no.5, pp.420 - 424-
dc.relation.isPartOfBIOTECHNOLOGY AND BIOPROCESS ENGINEERING-
dc.citation.titleBIOTECHNOLOGY AND BIOPROCESS ENGINEERING-
dc.citation.volume11-
dc.citation.number5-
dc.citation.startPage420-
dc.citation.endPage424-
dc.type.rimsART-
dc.type.docTypeArticle-
dc.identifier.kciidART001212144-
dc.description.journalClass1-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.description.journalRegisteredClasskci-
dc.relation.journalResearchAreaBiotechnology & Applied Microbiology-
dc.relation.journalWebOfScienceCategoryBiotechnology & Applied Microbiology-
dc.subject.keywordPlusSITE-SPECIFIC INCORPORATION-
dc.subject.keywordPlusNONNATURAL AMINO-ACIDS-
dc.subject.keywordPlusGENETIC-CODE-
dc.subject.keywordPlusSYNTHESIS SYSTEM-
dc.subject.keywordPlusCOLI-
dc.subject.keywordPlusCODONS-
dc.subject.keywordPlusPROBES-
dc.subject.keywordAuthorcell-free protein synthesis-
dc.subject.keywordAuthorunnatural amino acid-
dc.subject.keywordAuthorin vitro suppression-
dc.subject.keywordAuthortRNA-
dc.subject.keywordAuthorchloramphenicol-
dc.subject.keywordAuthoracetyltransferase-
dc.subject.keywordAuthorerythropoietin-
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