Recent advances in DNA sequencing by End-Labeled Free-Solution Electrophoresis (ELFSE)
DC Field | Value | Language |
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dc.contributor.author | Won, Jong-In | - |
dc.date.accessioned | 2022-02-07T05:42:19Z | - |
dc.date.available | 2022-02-07T05:42:19Z | - |
dc.date.created | 2022-02-07 | - |
dc.date.issued | 2006-05 | - |
dc.identifier.issn | 1226-8372 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/24561 | - |
dc.description.abstract | End-Labeled Free-Solution Electrophoresis (ELFSE) is a new technique that is a promising bioconjugate method for DNA sequencing (or separation) and genotyping by both capillary and microfluidic device electrophoresis. Because ELFSE enables high-resolution electrophoretic separation in aqueous buffer alone (i.e., without a polymer matrix), it eliminates the need to load viscous polymer networks into electrophoresis microchannels. To achieve microchannel DNA separations with high performance, ELFSE requires monodisperse perturbing entities (i.e., drag-tags), which create a large amount of frictional drag when pulled behind DNA during free-solution electrophoresis, and which have other properties suitable for microchannel electrophoresis. In this article, the theoretical concepts of ELFSE and the required characteristics of the drag-tag molecules for the ultimate performance of ELFSE are reviewed. Additionally, the merits and limitations of current drag-tags are also discussed in the context of recent experimental data of ELFSE separation (or sequencing). | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING | - |
dc.subject | SOLUTION CAPILLARY-ELECTROPHORESIS | - |
dc.subject | ARRAY ELECTROPHORESIS | - |
dc.subject | POLYACRYLAMIDE-GEL | - |
dc.subject | MOLECULAR-WEIGHT | - |
dc.subject | NUCLEIC-ACIDS | - |
dc.subject | DRAG-TAGS | - |
dc.subject | SEPARATIONS | - |
dc.subject | POLYMERS | - |
dc.subject | BASES | - |
dc.subject | MOBILITY | - |
dc.title | Recent advances in DNA sequencing by End-Labeled Free-Solution Electrophoresis (ELFSE) | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Won, Jong-In | - |
dc.identifier.doi | 10.1007/BF02932028 | - |
dc.identifier.scopusid | 2-s2.0-33745616505 | - |
dc.identifier.wosid | 000238768600001 | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.11, no.3, pp.179 - 186 | - |
dc.relation.isPartOf | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.citation.title | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.citation.volume | 11 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 179 | - |
dc.citation.endPage | 186 | - |
dc.type.rims | ART | - |
dc.type.docType | Review | - |
dc.identifier.kciid | ART001012342 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | SOLUTION CAPILLARY-ELECTROPHORESIS | - |
dc.subject.keywordPlus | ARRAY ELECTROPHORESIS | - |
dc.subject.keywordPlus | POLYACRYLAMIDE-GEL | - |
dc.subject.keywordPlus | MOLECULAR-WEIGHT | - |
dc.subject.keywordPlus | NUCLEIC-ACIDS | - |
dc.subject.keywordPlus | DRAG-TAGS | - |
dc.subject.keywordPlus | SEPARATIONS | - |
dc.subject.keywordPlus | POLYMERS | - |
dc.subject.keywordPlus | BASES | - |
dc.subject.keywordPlus | MOBILITY | - |
dc.subject.keywordAuthor | DNA sequencing | - |
dc.subject.keywordAuthor | capillary electrophoresis (CE) | - |
dc.subject.keywordAuthor | microfluidic device | - |
dc.subject.keywordAuthor | ELFSE | - |
dc.subject.keywordAuthor | drag-tag | - |
dc.subject.keywordAuthor | microchannel electrophoresis | - |
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