Comblike, monodisperse polypeptoid drag-tags for DNA separations by end-labeled free-solution electrophoresis (ELFSE)
DC Field | Value | Language |
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dc.contributor.author | Haynes, R.D. | - |
dc.contributor.author | Meagher, R.J. | - |
dc.contributor.author | Won, J.-I. | - |
dc.contributor.author | Bogdan, F.M. | - |
dc.contributor.author | Barron, A.E. | - |
dc.date.accessioned | 2022-02-17T05:42:40Z | - |
dc.date.available | 2022-02-17T05:42:40Z | - |
dc.date.created | 2022-02-17 | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 1043-1802 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/25678 | - |
dc.description.abstract | The development of innovative technologies designed to reduce the cost and increase the throughput of DNA separations continues to be important for large-scale sequencing and genotyping efforts. We report research aimed at the further development of a free-solution bioconjugate method of DNA size separation by capillary electrophoresis (CE), in particular, the determination of an optimal molecular architecture for polyamide-based drag-tags. We synthesized several branched poly(N-methoxyethyl glycine)s (poly(NMEG)s, a class of polypeptoids) as novel friction-generating entities for end-on attachment to DNA molecules. A 30-mer poly(NMEG) backbone, comprising five evenly spaced reactive ε-amino groups, was synthesized on solid phase, cleaved, and purified to monodispersity by RP-HPLC. Three different comblike derivatives of this backbone molecule were created by (1) acetylating the ε-amino groups or (2) appending small, monodisperse NMEG oligomers (a tetramer and an octamer). Grafting of the oligo(NMEG)s was done using solution-phase amide bond formation chemistry. Once purified to total monodispersity, the three different drag-tags were studied by free-solution electrophoresis to observe the effect of branching on their hydrodynamic drag or α and hence their ability to separate DNA. Drag was found to scale linearly with total molecular weight, regardless of branch length. The octamer-branched drag-tag-DNA conjugate was used to separate ssDNA products of 50, 75, 100, and 150 bases in length by free-solution CE in less than 10 min. Hence, the use of branched or comblike drag-tags is both a feasible and an effective way to achieve high frictional drag, allowing the high-resolution separation of relatively large DNA molecules by free-solution CE without the need to synthesize very long polymers. © 2005 American Chemical Society. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.title | Comblike, monodisperse polypeptoid drag-tags for DNA separations by end-labeled free-solution electrophoresis (ELFSE) | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Won, J.-I. | - |
dc.identifier.doi | 10.1021/bc0496915 | - |
dc.identifier.scopusid | 2-s2.0-22644438263 | - |
dc.identifier.bibliographicCitation | Bioconjugate Chemistry, v.16, no.4, pp.929 - 938 | - |
dc.relation.isPartOf | Bioconjugate Chemistry | - |
dc.citation.title | Bioconjugate Chemistry | - |
dc.citation.volume | 16 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 929 | - |
dc.citation.endPage | 938 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scopus | - |
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