Fed-batch production of D-ribose from sugar mixtures by transketolase-deficient Bacillus subtilis SPK1
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, YC | - |
dc.contributor.author | Kim, SG | - |
dc.contributor.author | Park, K | - |
dc.contributor.author | Lee, KH | - |
dc.contributor.author | Seo, JH | - |
dc.date.accessioned | 2022-02-18T07:40:51Z | - |
dc.date.available | 2022-02-18T07:40:51Z | - |
dc.date.created | 2022-02-18 | - |
dc.date.issued | 2004-12 | - |
dc.identifier.issn | 0175-7598 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/25719 | - |
dc.description.abstract | D-Ribose, a five-carbon sugar, is used as a key intermediate for the production of various biomaterials, such as riboflavin and inosine monophosphate. A high D-ribose-producing Bacillus subtilis SPK1 strain was constructed by the chemical mutation of the transketolase-deficient strain, B. subtilis JY1. Batch fermentation of B. subtilis SPK1 with 20 g l(-1) xylose and 20 g l(-1) glucose resulted in 4.78 g l(-1) dry cell mass, 23.0 g l(-1) D-ribose concentration, and 0.72 g l(-1) h(-1) productivity, corresponding to a 1.5- to 1.7-fold increase when compared with values for the parental strain. A late-exponential phase was chosen as the best point for switching to a fed-batch process. Optimized fed-batch fermentation of B. subtilis SPK1, feeding a mixture of 200 g l(-1) xylose and 50 g l(-1) glucose after the late-exponential phase reduced the residual xylose and glucose concentrations to less than 7.0 g l(-1) and gave the best results of 46.6 g l(-1) D-ribose concentration and 0.88 g l(-1) h(-1) productivity which were 2.0- and 1.2-fold higher than the corresponding values in a simple batch fermentation. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER | - |
dc.subject | MUTANT STRAIN | - |
dc.subject | GLUCOSE | - |
dc.subject | OPTIMIZATION | - |
dc.subject | FERMENTATION | - |
dc.title | Fed-batch production of D-ribose from sugar mixtures by transketolase-deficient Bacillus subtilis SPK1 | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, K | - |
dc.identifier.doi | 10.1007/s00253-004-1678-3 | - |
dc.identifier.scopusid | 2-s2.0-11244261427 | - |
dc.identifier.wosid | 000225225300010 | - |
dc.identifier.bibliographicCitation | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.66, no.3, pp.297 - 302 | - |
dc.relation.isPartOf | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.title | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 66 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 297 | - |
dc.citation.endPage | 302 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | MUTANT STRAIN | - |
dc.subject.keywordPlus | GLUCOSE | - |
dc.subject.keywordPlus | OPTIMIZATION | - |
dc.subject.keywordPlus | FERMENTATION | - |
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