Development of a Spacer-optimized Quenchbody against Tumor Necrosis Factor Alpha
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 윤한울 | - |
dc.contributor.author | Hiroshi Ueda | - |
dc.contributor.author | 정희진 | - |
dc.date.accessioned | 2022-11-10T05:41:23Z | - |
dc.date.available | 2022-11-10T05:41:23Z | - |
dc.date.created | 2022-11-10 | - |
dc.date.issued | 2022-10-01 | - |
dc.identifier.issn | 1226-8372 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/30565 | - |
dc.description.abstract | Tumor necrosis factor alpha (TNFα) is a proinflammatory cytokine, and its overproduction causes infectious diseases and autoimmune disorders. Therefore, rapid and accurate detection of TNFα is important, and the need for a convenient detection system has increased. In this study, single-chain variable fragment (scFv)-type Quenchbody (Q-body) was developed that could detect TNFα by mixing the Q-body with TNFα, followed by measuring its fluorescence intensity. Recombinant scFvs were constructed with several different lengths of flexible GGGS repeat spacers between dye and scFv. The fluorescent responses of Q-bodies increased in an antigen-dosedependent manner. The fluorescence intensity in the presence of a maximum concentration of antigen was compared to that of the denatured Q-body, resulting in the Q-body with the (GGGS)5 spacer showing a fully de-quenched signal, whereas other Q-bodies with shorter spacer lengths showed insufficient de-quenching efficiency. These results indicated that adjusting the spacer length is important for improving the response of the Q-body. The Q-body with the optimized spacer length showed a maximum 4.5-fold increase in fluorescence intensity with a broad antigen concentration range, and nanomolar order of detection limit, indicating the usefulness of this Q-body for one-step detection of TNFα. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | 한국생물공학회 | - |
dc.title | Development of a Spacer-optimized Quenchbody against Tumor Necrosis Factor Alpha | - |
dc.title.alternative | Development of a Spacer-optimized Quenchbody against Tumor Necrosis Factor Alpha | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | 정희진 | - |
dc.identifier.doi | 10.1007/s12257-022-0088-7 | - |
dc.identifier.scopusid | 2-s2.0-85141056566 | - |
dc.identifier.wosid | 000877974700001 | - |
dc.identifier.bibliographicCitation | Biotechnology and Bioprocess Engineering, v.27, no.5, pp.820 - 830 | - |
dc.relation.isPartOf | Biotechnology and Bioprocess Engineering | - |
dc.citation.title | Biotechnology and Bioprocess Engineering | - |
dc.citation.volume | 27 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 820 | - |
dc.citation.endPage | 830 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002894649 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | TARGETING TNF-ALPHA | - |
dc.subject.keywordPlus | SINGLE-CHAIN FV | - |
dc.subject.keywordPlus | SCFV ANTIBODY | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | CYTOKINES | - |
dc.subject.keywordPlus | FRAGMENT | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | CONSTRUCTION | - |
dc.subject.keywordPlus | PATHOGENESIS | - |
dc.subject.keywordPlus | MACROPHAGES | - |
dc.subject.keywordAuthor | antibody fragment | - |
dc.subject.keywordAuthor | Quenchbody | - |
dc.subject.keywordAuthor | fluorescence immunosensor | - |
dc.subject.keywordAuthor | tumor necrosis factor alpha | - |
dc.subject.keywordAuthor | on-site immunoassay | - |
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