Generation of a novel antibody against BxPrx, a diagnostic marker of pine wilt disease
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, S.-H. | - |
dc.contributor.author | Yun, H.-Y. | - |
dc.contributor.author | Jeong, H.-J. | - |
dc.date.accessioned | 2023-03-21T01:41:46Z | - |
dc.date.available | 2023-03-21T01:41:46Z | - |
dc.date.issued | 2023-05-01 | - |
dc.identifier.issn | 0301-4851 | - |
dc.identifier.issn | 1573-4978 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/30952 | - |
dc.description.abstract | Background: Bursaphelenchus xylophilus is a pathogenic nematode that causes pine wilt disease (PWD). To prevent the rapid spread of this pathogen, developing a method for rapid and accurate detection of B. xylophilus is required. Methods and results: In this study, we produced a B. xylophilus peroxiredoxin (BxPrx), which is a protein that is overexpressed in B. xylophilus. Using recombinant BxPrx as an antigen, we generated and selected a novel antibody that binds to BxPrx via phage display and biopanning. We subcloned the anti-BxPrx single-chain variable fragment-encoding phagemid DNA to mammalian expression vector. We transfected the plasmid into mammalian cells and produced a highly sensitive recombinant antibody that enabled nanogram order detection of BxPrx. Conclusion: The sequence of anti-BxPrx antibody as well as the rapid immunoassay system described here can be applied for rapid and accurate diagnosis of PWD. © 2023, The Author(s), under exclusive licence to Springer Nature B.V. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Springer Science and Business Media B.V. | - |
dc.title | Generation of a novel antibody against BxPrx, a diagnostic marker of pine wilt disease | - |
dc.type | Article | - |
dc.publisher.location | 네델란드 | - |
dc.identifier.doi | 10.1007/s11033-023-08342-3 | - |
dc.identifier.scopusid | 2-s2.0-85149795782 | - |
dc.identifier.wosid | 000947251400001 | - |
dc.identifier.bibliographicCitation | Molecular Biology Reports, v.50, no.5, pp 4715 - 4721 | - |
dc.citation.title | Molecular Biology Reports | - |
dc.citation.volume | 50 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 4715 | - |
dc.citation.endPage | 4721 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.subject.keywordPlus | BURSAPHELENCHUS-XYLOPHILUS | - |
dc.subject.keywordPlus | WOOD NEMATODE | - |
dc.subject.keywordPlus | PEROXIREDOXIN | - |
dc.subject.keywordAuthor | Bursaphelenchus xylophilus | - |
dc.subject.keywordAuthor | Immunoassay | - |
dc.subject.keywordAuthor | Monoclonal antibody | - |
dc.subject.keywordAuthor | Phage display | - |
dc.subject.keywordAuthor | Pine wilt disease | - |
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