Generation of an scFv-type Quenchbody Against HER2 for Sensitive Detection of Human Breast Cancer
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Rizi Shima Yousefi | - |
dc.contributor.author | 김선희 | - |
dc.contributor.author | 이정훈 | - |
dc.contributor.author | 정희진 | - |
dc.date.accessioned | 2023-04-24T03:40:19Z | - |
dc.date.available | 2023-04-24T03:40:19Z | - |
dc.date.created | 2023-04-24 | - |
dc.date.issued | 2023-03 | - |
dc.identifier.issn | 1225-7117 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/31092 | - |
dc.description.abstract | The advanced clinical stage of breast cancer causes a lower survival rate even though the survival rate in the early stages is relatively high. Thus, the development of a rapid and easy-to-access diagnostic method is needed. In this study, a single chain Fv (scFv)-type antibody fragment that binds to HER2, a transmembrane protein overexpressed on breast cancer cells, was generated. The scFv was converted to a Quenchbody by site-specifically labeling the scFv using fluorescent dye through a maleimide-thiol reaction. The Quenchbody could detect a picomolar order of HER2 in a few minutes, and its signal-to-background ratio of fluorescence intensity was higher than a fluorescence scFv generated through a conventional fluorescent dye-conjugating method. The Quenchbody can be used as a probe for detecting and imaging of breast cancer cells for point-of-care diagnosis of breast cancer. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | 한국생물공학회 | - |
dc.title | Generation of an scFv-type Quenchbody Against HER2 for Sensitive Detection of Human Breast Cancer | - |
dc.title.alternative | Generation of an scFv-type Quenchbody Against HER2 for Sensitive Detection of Human Breast Cancer | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | 정희진 | - |
dc.identifier.doi | 10.7841/ksbbj.2023.38.1.35 | - |
dc.identifier.bibliographicCitation | KSBB Journal, v.38, no.1, pp.35 - 45 | - |
dc.relation.isPartOf | KSBB Journal | - |
dc.citation.title | KSBB Journal | - |
dc.citation.volume | 38 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 35 | - |
dc.citation.endPage | 45 | - |
dc.type.rims | ART | - |
dc.identifier.kciid | ART002950941 | - |
dc.description.journalClass | 2 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | kci | - |
dc.subject.keywordAuthor | breast cancer | - |
dc.subject.keywordAuthor | human epidermal growth factor receptor 2 | - |
dc.subject.keywordAuthor | antibody fragment | - |
dc.subject.keywordAuthor | quenchbody | - |
dc.subject.keywordAuthor | immunoassay | - |
dc.subject.keywordAuthor | onepot detection | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
94, Wausan-ro, Mapo-gu, Seoul, 04066, Korea02-320-1314
COPYRIGHT 2020 HONGIK UNIVERSITY. ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.