Complementation of reducing power for 5-hydroxyvaleric acid and 1,5-pentanediol production via glucose dehydrogenase in Escherichia coli whole-cell system
DC Field | Value | Language |
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dc.contributor.author | Kim, Byungchan | - |
dc.contributor.author | Oh, Suk Jin | - |
dc.contributor.author | Hwang, Jeong Hyeon | - |
dc.contributor.author | Kim, Hyun Jin | - |
dc.contributor.author | Shin, Nara | - |
dc.contributor.author | Joo, Jeong Chan | - |
dc.contributor.author | Choi, Kwon-Young | - |
dc.contributor.author | Park, See-Hyoung | - |
dc.contributor.author | Park, Kyungmoon | - |
dc.contributor.author | Bhatia, Shashi Kant | - |
dc.contributor.author | Yang, Yung-Hun | - |
dc.date.accessioned | 2023-08-31T01:40:20Z | - |
dc.date.available | 2023-08-31T01:40:20Z | - |
dc.date.issued | 2023-10 | - |
dc.identifier.issn | 0141-0229 | - |
dc.identifier.issn | 1879-0909 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/31583 | - |
dc.description.abstract | One of the key intermediates, 5-hydroxyvaleric acid (5-HV), is used in the synthesis of polyhydroxyalkanoate monomer, δ-valerolactone, 1,5-pentanediol (1,5-PDO), and many other substances. Due to global environmental problems, eco-friendly bio-based synthesis of various platform chemicals and key intermediates are socially required, but few previous studies on 5-HV biosynthesis have been conducted. To establish a sustainable bioprocess for 5-HV production, we introduced gabT encoding 4-aminobutyrate aminotransferase and yqhD encoding alcohol dehydrogenase to produce 5-HV from 5-aminovaleric acid (5-AVA), through glutarate semialdehyde in Escherichia coli whole-cell reaction. As, high reducing power is required to produce high concentrations of 5-HV, we newly introduced glucose dehydrogenase (GDH) for NADPH regeneration system from Bacillus subtilis 168. By applying GDH with D-glucose and optimizing the parameters, 5-HV conversion rate from 5-AVA increased from 47% (w/o GDH) to 82% when using 200 mM (23.4 g/L) of 5-AVA. Also, it reached 56% conversion in 2 h, showing 56 mM/h (6.547 g/L/h) productivity from 200 mM 5-AVA, finally reaching 350 mM (41 g/L) and 14.6 mM/h (1.708 g/L/h) productivity at 24 h when 1 M (117.15 g/L) 5-AVA was used. When the whole-cell system with GDH was expanded to produce 1,5-PDO, its production was also increased 5-fold. Considering that 5-HV and 1,5-PDO production depends heavily on the reducing power of the cells, we successfully achieved a significant increase in 5-HV and 1,5-PDO production using GDH. © 2023 Elsevier Inc. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Elsevier Inc. | - |
dc.title | Complementation of reducing power for 5-hydroxyvaleric acid and 1,5-pentanediol production via glucose dehydrogenase in Escherichia coli whole-cell system | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1016/j.enzmictec.2023.110305 | - |
dc.identifier.scopusid | 2-s2.0-85168544771 | - |
dc.identifier.wosid | 001060843600001 | - |
dc.identifier.bibliographicCitation | Enzyme and Microbial Technology, v.170 | - |
dc.citation.title | Enzyme and Microbial Technology | - |
dc.citation.volume | 170 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordAuthor | 1,5-pentanediol | - |
dc.subject.keywordAuthor | 5-hydroxyvaleric acid | - |
dc.subject.keywordAuthor | Glucose dehydrogenase | - |
dc.subject.keywordAuthor | Recombinant E. coli | - |
dc.subject.keywordAuthor | Whole-cell biocatalysis | - |
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