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Complementation of reducing power for 5-hydroxyvaleric acid and 1,5-pentanediol production via glucose dehydrogenase in Escherichia coli whole-cell system

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dc.contributor.authorKim, Byungchan-
dc.contributor.authorOh, Suk Jin-
dc.contributor.authorHwang, Jeong Hyeon-
dc.contributor.authorKim, Hyun Jin-
dc.contributor.authorShin, Nara-
dc.contributor.authorJoo, Jeong Chan-
dc.contributor.authorChoi, Kwon-Young-
dc.contributor.authorPark, See-Hyoung-
dc.contributor.authorPark, Kyungmoon-
dc.contributor.authorBhatia, Shashi Kant-
dc.contributor.authorYang, Yung-Hun-
dc.date.accessioned2023-08-31T01:40:20Z-
dc.date.available2023-08-31T01:40:20Z-
dc.date.issued2023-10-
dc.identifier.issn0141-0229-
dc.identifier.issn1879-0909-
dc.identifier.urihttps://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/31583-
dc.description.abstractOne of the key intermediates, 5-hydroxyvaleric acid (5-HV), is used in the synthesis of polyhydroxyalkanoate monomer, δ-valerolactone, 1,5-pentanediol (1,5-PDO), and many other substances. Due to global environmental problems, eco-friendly bio-based synthesis of various platform chemicals and key intermediates are socially required, but few previous studies on 5-HV biosynthesis have been conducted. To establish a sustainable bioprocess for 5-HV production, we introduced gabT encoding 4-aminobutyrate aminotransferase and yqhD encoding alcohol dehydrogenase to produce 5-HV from 5-aminovaleric acid (5-AVA), through glutarate semialdehyde in Escherichia coli whole-cell reaction. As, high reducing power is required to produce high concentrations of 5-HV, we newly introduced glucose dehydrogenase (GDH) for NADPH regeneration system from Bacillus subtilis 168. By applying GDH with D-glucose and optimizing the parameters, 5-HV conversion rate from 5-AVA increased from 47% (w/o GDH) to 82% when using 200 mM (23.4 g/L) of 5-AVA. Also, it reached 56% conversion in 2 h, showing 56 mM/h (6.547 g/L/h) productivity from 200 mM 5-AVA, finally reaching 350 mM (41 g/L) and 14.6 mM/h (1.708 g/L/h) productivity at 24 h when 1 M (117.15 g/L) 5-AVA was used. When the whole-cell system with GDH was expanded to produce 1,5-PDO, its production was also increased 5-fold. Considering that 5-HV and 1,5-PDO production depends heavily on the reducing power of the cells, we successfully achieved a significant increase in 5-HV and 1,5-PDO production using GDH. © 2023 Elsevier Inc.-
dc.language영어-
dc.language.isoENG-
dc.publisherElsevier Inc.-
dc.titleComplementation of reducing power for 5-hydroxyvaleric acid and 1,5-pentanediol production via glucose dehydrogenase in Escherichia coli whole-cell system-
dc.typeArticle-
dc.publisher.location미국-
dc.identifier.doi10.1016/j.enzmictec.2023.110305-
dc.identifier.scopusid2-s2.0-85168544771-
dc.identifier.wosid001060843600001-
dc.identifier.bibliographicCitationEnzyme and Microbial Technology, v.170-
dc.citation.titleEnzyme and Microbial Technology-
dc.citation.volume170-
dc.type.docTypeArticle-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.relation.journalResearchAreaBiotechnology & Applied Microbiology-
dc.relation.journalWebOfScienceCategoryBiotechnology & Applied Microbiology-
dc.subject.keywordAuthor1,5-pentanediol-
dc.subject.keywordAuthor5-hydroxyvaleric acid-
dc.subject.keywordAuthorGlucose dehydrogenase-
dc.subject.keywordAuthorRecombinant E. coli-
dc.subject.keywordAuthorWhole-cell biocatalysis-
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