Generation of fluorescent minibodies for rapid FLISA-based detection of pine wilt disease marker
DC Field | Value | Language |
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dc.contributor.author | Kim, Seon-Hyung | - |
dc.contributor.author | Jeong, Hee-Jin | - |
dc.date.accessioned | 2023-11-22T06:40:19Z | - |
dc.date.available | 2023-11-22T06:40:19Z | - |
dc.date.issued | 2024-02 | - |
dc.identifier.issn | 0268-2575 | - |
dc.identifier.issn | 1097-4660 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/31862 | - |
dc.description.abstract | BACKGROUND: Development of a rapid and accurate detection method for a pine wilt disease (PWD) biomarker is crucial to prevent the spread of this disease in forests. In this study, we produced a recombinant minibody-type antibody against Bursaphelenchus xylophilus peroxiredoxin (BxPrx), which is a protein secreted by the PWD-causing B. xylophilus pathogen. Results: We labeled the anti-BxPrx minibody using two different fluorescent dyes, Alexa Fluor (AF) 488 and AF647, and established a fluorescence-linked immunosorbent assay (FLISA) system using these probes. Both fluorescent minibodies showed limit of detection values of 10.2 and 14.6 ng for the AF488- and AF647-labeled antibodies, respectively, suggesting the usefulness of these probes for FLISA-based sensitive detection of BxPrx. Conclusion: The entire duration for performing FLISA was 3 h, and enzyme-conjugated secondary antibody incubation, washing and enzymatic reaction steps were not required, which was comparable to traditional ELISA. Both AF488- and AF647-labeled minibodies showed high sensitivity, suggesting that these probes are useful for various fluorescence measurement techniques, which typically use green or red filters. © 2023 Society of Chemical Industry (SCI). © 2023 Society of Chemical Industry (SCI). | - |
dc.format.extent | 4 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | John Wiley and Sons Ltd | - |
dc.title | Generation of fluorescent minibodies for rapid FLISA-based detection of pine wilt disease marker | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1002/jctb.7539 | - |
dc.identifier.scopusid | 2-s2.0-85176569450 | - |
dc.identifier.wosid | 001099019800001 | - |
dc.identifier.bibliographicCitation | Journal of Chemical Technology and Biotechnology, v.99, no.2, pp 381 - 384 | - |
dc.citation.title | Journal of Chemical Technology and Biotechnology | - |
dc.citation.volume | 99 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 381 | - |
dc.citation.endPage | 384 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Engineering, Environmental | - |
dc.relation.journalWebOfScienceCategory | Engineering, Chemical | - |
dc.subject.keywordPlus | BURSAPHELENCHUS-XYLOPHILUS | - |
dc.subject.keywordPlus | PEROXIREDOXIN | - |
dc.subject.keywordPlus | NEMATODE | - |
dc.subject.keywordAuthor | BxPrx | - |
dc.subject.keywordAuthor | FLISA | - |
dc.subject.keywordAuthor | fluorescent immunoassay | - |
dc.subject.keywordAuthor | minibody | - |
dc.subject.keywordAuthor | pine wilt disease | - |
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