Development of fluorescence-linked immunosorbent assay for rapid detection of <i>Staphylococcus aureus</i>
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Joo-Kyung | - |
dc.contributor.author | Yun, Hyun-Young | - |
dc.contributor.author | Kim, Jae-Seok | - |
dc.contributor.author | Kim, Wooseong | - |
dc.contributor.author | Lee, Chang-Soo | - |
dc.contributor.author | Kim, Byung-Gee | - |
dc.contributor.author | Jeong, Hee-Jin | - |
dc.date.accessioned | 2024-01-29T05:00:30Z | - |
dc.date.available | 2024-01-29T05:00:30Z | - |
dc.date.issued | 2024-12 | - |
dc.identifier.issn | 0175-7598 | - |
dc.identifier.issn | 1432-0614 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/32608 | - |
dc.description.abstract | Staphylococcus aureus is a major pathogen that causes infections and life-threatening diseases. Although antibiotics, such as methicillin, have been used, methicillin-resistant S. aureus (MRSA) causes high morbidity and mortality rates, and conventional detection methods are difficult to be used because of time-consuming process. To control the spread of S. aureus, a development of a rapid and simple detection method is required. In this study, we generated a fluorescent anti-S. aureus antibody, and established a novel fluorescence-linked immunosorbent assay (FLISA)-based S. aureus detection method. The method showed high sensitivity and low limit of detection toward MRSA detection. The assay time for FLISA was 5 h, which was faster than that of conventional enzyme-linked immunosorbent assay (ELISA) or rapid ELISA. Moreover, the FLISA-based detection method was applied to diagnose clinically isolated MRSA samples that required only 5.3 h of preincubation. The FLISA method developed in this study can be widely applied as a useful tool for convenient S. aureus detection. | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | SPRINGER | - |
dc.title | Development of fluorescence-linked immunosorbent assay for rapid detection of <i>Staphylococcus aureus</i> | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1007/s00253-023-12836-2 | - |
dc.identifier.scopusid | 2-s2.0-85180710127 | - |
dc.identifier.wosid | 001132530600001 | - |
dc.identifier.bibliographicCitation | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.108, no.1, pp 1 - 10 | - |
dc.citation.title | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 108 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 10 | - |
dc.type.docType | Review | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | LATEX AGGLUTINATION-TEST | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | BACTERIA | - |
dc.subject.keywordPlus | MRSA | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | TIME | - |
dc.subject.keywordAuthor | Staphylococcus aureus | - |
dc.subject.keywordAuthor | Fluorescent antibody | - |
dc.subject.keywordAuthor | Immunoassay | - |
dc.subject.keywordAuthor | FLISA | - |
dc.subject.keywordAuthor | ELISA | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
94, Wausan-ro, Mapo-gu, Seoul, 04066, Korea02-320-1314
COPYRIGHT 2020 HONGIK UNIVERSITY. ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.