Characterization of ELP-fused -Transaminase and Its Application for the Biosynthesis of -Amino Acid
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jeon, Hyunwoo | - |
dc.contributor.author | Sarak, Sharad | - |
dc.contributor.author | Lee, Sang-Hyuk | - |
dc.contributor.author | Bea, Han-Seop | - |
dc.contributor.author | Patil, Mahesh | - |
dc.contributor.author | Kim, Geon-Hee | - |
dc.contributor.author | Kim, Byung-Gee | - |
dc.contributor.author | Won, Jong In | - |
dc.contributor.author | Yun, Hyungdon | - |
dc.date.available | 2020-07-10T04:19:21Z | - |
dc.date.created | 2020-07-06 | - |
dc.date.issued | 2018-09 | - |
dc.identifier.issn | 1226-8372 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/3278 | - |
dc.description.abstract | Optically pure amines, -amino acids and -amino acids are the valuable precursors to produce biologically active compounds. The -TAs are the class of enzymes which are widely used to produce such compounds. In this work (S)--transaminase from the thermophilic eubacterium Sphaerobacter thermophilus (St-TA) was fused with Elastin-like polypeptides (ELPs) through the cloning process and expressed in E. coli cells. The characterization of this fusion complex was performed with respect to thermostability and effect of DMSO. Where in case of St-TA-ELP-V-60, major difference in the transition temperature (T-t) was observed, wherein a T-t of 38 and 70 degrees C was observed at the increasing concentration of DMSO from 5 to 25% (v/v). Interestingly, these fusion proteins the activity was preserved even after the aggregation of fusion complex at Tt. The substrate specificity and product inhibition analysis showed that -TA-ELPs had comparable results as that of wild type -TA. Moreover, the fused -TA could be efficiently reused for up to 20 batches of transamination reaction. Furthermore, the applicability of the fusion protein for the production of a sitagliptin precursor (R)-3-amino-4-(2,4,5-triflurophenyl) butanoic acid (3-ATfBA) was evaluated, wherein 3-ATfBA was synthesized with good conversion (65%). | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING | - |
dc.subject | ELASTIN-LIKE POLYPEPTIDE | - |
dc.subject | PROTEIN-BASED POLYMERS | - |
dc.subject | OMEGA-TRANSAMINASE | - |
dc.subject | ENZYMATIC-SYNTHESIS | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | PURIFICATION | - |
dc.subject | IMMOBILIZATION | - |
dc.subject | EXPRESSION | - |
dc.subject | AMINATION | - |
dc.subject | SUPPORT | - |
dc.title | Characterization of ELP-fused -Transaminase and Its Application for the Biosynthesis of -Amino Acid | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Won, Jong In | - |
dc.identifier.doi | 10.1007/s12257-018-0268-7 | - |
dc.identifier.scopusid | 2-s2.0-85056273415 | - |
dc.identifier.wosid | 000449943200001 | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.23, no.5, pp.481 - 489 | - |
dc.relation.isPartOf | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.citation.title | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.citation.volume | 23 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 481 | - |
dc.citation.endPage | 489 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002403075 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | ELASTIN-LIKE POLYPEPTIDE | - |
dc.subject.keywordPlus | PROTEIN-BASED POLYMERS | - |
dc.subject.keywordPlus | OMEGA-TRANSAMINASE | - |
dc.subject.keywordPlus | ENZYMATIC-SYNTHESIS | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | IMMOBILIZATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | AMINATION | - |
dc.subject.keywordPlus | SUPPORT | - |
dc.subject.keywordAuthor | (S)-omega-transaminase | - |
dc.subject.keywordAuthor | elastin-like polypeptides | - |
dc.subject.keywordAuthor | chiral amines | - |
dc.subject.keywordAuthor | bio-catalysis | - |
dc.subject.keywordAuthor | thermostability | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
94, Wausan-ro, Mapo-gu, Seoul, 04066, Korea02-320-1314
COPYRIGHT 2020 HONGIK UNIVERSITY. ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.