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Soluble expression of recombinant coagulation factor IX protein using Escherichia coli
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| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Park, Byoung-Hee | - |
| dc.contributor.author | Yun, Hanool | - |
| dc.contributor.author | Jeong, Hee-Jin | - |
| dc.date.accessioned | 2024-04-25T04:00:20Z | - |
| dc.date.available | 2024-04-25T04:00:20Z | - |
| dc.date.issued | 2024-07 | - |
| dc.identifier.issn | 2405-5808 | - |
| dc.identifier.issn | 2405-5808 | - |
| dc.identifier.uri | https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/33045 | - |
| dc.description.abstract | Hemophilia B is a congenital bleeding disorder caused by factor IX (FIX) deficiency. Generation of recombinant FIX (rFIX) is required for detecting a Hemophilia B indicator, anti-FIX antibody. In this study, we described a method for producing recombinant FIX (rFIX) using Escherichia coli. We constructed a FIX-expressing plasmid without a fusion tag protein-encoding gene and produced rFIX as a soluble form within five days. Dose-dependent curve was obtained from ELISA using anti-FIX antibody, indicating that the rFIX can be used as an antigen to detect anti-FIX antibody with high affinity and sensitivity. © 2024 The Authors | - |
| dc.language | 영어 | - |
| dc.language.iso | ENG | - |
| dc.publisher | Elsevier B.V. | - |
| dc.title | Soluble expression of recombinant coagulation factor IX protein using Escherichia coli | - |
| dc.type | Article | - |
| dc.publisher.location | 네델란드 | - |
| dc.identifier.doi | 10.1016/j.bbrep.2024.101714 | - |
| dc.identifier.scopusid | 2-s2.0-85190499466 | - |
| dc.identifier.bibliographicCitation | Biochemistry and Biophysics Reports, v.38 | - |
| dc.citation.title | Biochemistry and Biophysics Reports | - |
| dc.citation.volume | 38 | - |
| dc.type.docType | Article | - |
| dc.description.isOpenAccess | Y | - |
| dc.description.journalRegisteredClass | scopus | - |
| dc.description.journalRegisteredClass | esci | - |
| dc.subject.keywordAuthor | Escherichia coli | - |
| dc.subject.keywordAuthor | Factor IX | - |
| dc.subject.keywordAuthor | Hemophilia | - |
| dc.subject.keywordAuthor | Protein expression | - |
| dc.subject.keywordAuthor | Recombinant protein | - |
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Related Researcher
- Jeong, Hee-Jin
- Science & Technology (Biological and Chemical Engineering)