Separation efficiency of free-solution conjugated electrophoresis with drag-tags incorporating a synthetic amino acid
DC Field | Value | Language |
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dc.contributor.author | Seo, Kyung-Ho | - |
dc.contributor.author | Chu, Hun-Su | - |
dc.contributor.author | Yoo, Tae Hyeon | - |
dc.contributor.author | Lee, Sun-Gu | - |
dc.contributor.author | Won, Jong-In | - |
dc.date.available | 2020-07-10T06:12:46Z | - |
dc.date.created | 2020-07-06 | - |
dc.date.issued | 2016-03 | - |
dc.identifier.issn | 0173-0835 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/8056 | - |
dc.description.abstract | DNA sequencing or separation by conventional capillary electrophoresis with a polymer matrix has some inherent drawbacks, such as the expense of polymer matrix and limitations in sequencing read length. As DNA fragments have a linear charge-to-friction ratio in free solution, DNA fragments cannot be separated by size. However, size-based separation of DNA is possible in free-solution conjugate electrophoresis (FSCE) if a "drag-tag" is attached to DNA fragments because the tag breaks the linear charge-to-friction scaling. Although several previous studies have demonstrated the feasibility of DNA separation by free-solution conjugated electrophoresis, generation of a monodisperse drag-tag and identification of a strong, site-specific conjugation method between a DNA fragment and a drag-tag are challenges that still remain. In this study, we demonstrate an efficient FSCE method by conjugating a biologically synthesized elastin-like polypeptide (ELP) and green fluorescent protein (GFP) to DNA fragments. In addition, to produce strong and site-specific conjugation, a methionine residue in drag-tags is replaced with homopropargylglycine (Hpg), which can be conjugated specifically to a DNA fragment with an azide site. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.subject | SOLUTION CAPILLARY-ELECTROPHORESIS | - |
dc.subject | RECOMBINANT PROTEINS | - |
dc.subject | DNA | - |
dc.subject | SYSTEM | - |
dc.title | Separation efficiency of free-solution conjugated electrophoresis with drag-tags incorporating a synthetic amino acid | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Won, Jong-In | - |
dc.identifier.doi | 10.1002/elps.201500506 | - |
dc.identifier.scopusid | 2-s2.0-84975780768 | - |
dc.identifier.wosid | 000372947700017 | - |
dc.identifier.bibliographicCitation | ELECTROPHORESIS, v.37, no.5-6, pp.818 - 825 | - |
dc.relation.isPartOf | ELECTROPHORESIS | - |
dc.citation.title | ELECTROPHORESIS | - |
dc.citation.volume | 37 | - |
dc.citation.number | 5-6 | - |
dc.citation.startPage | 818 | - |
dc.citation.endPage | 825 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.subject.keywordPlus | SOLUTION CAPILLARY-ELECTROPHORESIS | - |
dc.subject.keywordPlus | RECOMBINANT PROTEINS | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordAuthor | Drag-tag | - |
dc.subject.keywordAuthor | ELP | - |
dc.subject.keywordAuthor | FSCE | - |
dc.subject.keywordAuthor | GFP | - |
dc.subject.keywordAuthor | Synthetic amino acid | - |
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