Optimization of Direct Lysine Decarboxylase Biotransformation for Cadaverine Production with Whole-Cell Biocatalysts at High Lysine Concentration
DC Field | Value | Language |
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dc.contributor.author | Kim, Hyun Joong | - |
dc.contributor.author | Kim, Yong Hyun | - |
dc.contributor.author | Shin, Ji-Hyun | - |
dc.contributor.author | Bhatia, Shashi Kant | - |
dc.contributor.author | Sathiyanarayanan, Ganesan | - |
dc.contributor.author | Seo, Hyung-Min | - |
dc.contributor.author | Choi, Kwon Young | - |
dc.contributor.author | Yang, Yung-Hun | - |
dc.contributor.author | Park, Kyungmoon | - |
dc.date.available | 2020-07-10T07:03:19Z | - |
dc.date.created | 2020-07-06 | - |
dc.date.issued | 2015-07 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/9673 | - |
dc.description.abstract | Cadaverine (1,5-diaminopentane) is an important industrial chemical with a wide range of applications. Although there have been many efforts to produce cadaverine through fermentation, there are not many reports of the direct cadaverine production from lysine using biotransformation. Whole-cell reactions were examined using a recombinant Escherichia coli strain overexpressing the E. coli MG1655 cadA gene, and various parameters were investigated for the whole-cell bioconversion of lysine to cadaverine. A high concentration of lysine resulted in the synthesis of pyridoxa1-5'-phosphate (PLP) and it was found to be a critical control factor for the biotransformation of lysine to cadaverine. When 0.025 mM PLP and 1.75 M lysine in 500 mM sodium acetate buffer (pH6) were used, consumption of 91% lysine and conversion of about 80% lysine to cadaverine were successfully achieved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | CORYNEBACTERIUM-GLUTAMICUM | - |
dc.subject | GENE | - |
dc.title | Optimization of Direct Lysine Decarboxylase Biotransformation for Cadaverine Production with Whole-Cell Biocatalysts at High Lysine Concentration | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Kyungmoon | - |
dc.identifier.doi | 10.4014/jmb.1412.12052 | - |
dc.identifier.scopusid | 2-s2.0-84937414428 | - |
dc.identifier.wosid | 000358701800016 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.25, no.7, pp.1108 - 1113 | - |
dc.relation.isPartOf | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 25 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 1108 | - |
dc.citation.endPage | 1113 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002014182 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | CORYNEBACTERIUM-GLUTAMICUM | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordAuthor | Biotransformation | - |
dc.subject.keywordAuthor | whole-cell biocatalyst | - |
dc.subject.keywordAuthor | lysine decarboxylase | - |
dc.subject.keywordAuthor | cadaverine | - |
dc.subject.keywordAuthor | high-concentration reaction | - |
dc.subject.keywordAuthor | Escherichia coli | - |
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