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Autoclave treatment fails to completely inactivate DLB alpha-synuclein seeding activityopen access
- Authors
- Han, Jung-Youn; Park, Kyung-Je; Park, Hoo-Chang; Lee, Yu-Ran; Moore, Roger A.; Sohn, Hyun-Joo; Choi, Young Pyo
- Issue Date
- Jul-2023
- Publisher
- Elsevier BV
- Keywords
- Alpha-synuclein; Seeds; Inactivation; Autoclave; RT-QuIC; Prion
- Citation
- Biochemistry and Biophysics Reports, v.34
- Journal Title
- Biochemistry and Biophysics Reports
- Volume
- 34
- ISSN
- 2405-5808
- Abstract
- Synucleinopathies are characterized by the deposition of alpha-synuclein (alpha-syn) aggregates in brain tissue. Pathological alpha-syn aggregates propagate in a prion-like manner and display prion-like biochemical properties. Using RT-QuIC, we measured alpha-syn seeding activity from brains of Dementia with Lewy body (DLB) patients post autoclave. Here, we show that autoclaving at 121 degrees C removes one to two log(10) of alpha-syn seeding activity but the remaining 50% seeding dose (SD50) is more than 10(7)/mg tissue. DLB brain samples autoclaved at 132 degrees C still revealed an SD50 of approximately 10(6)/mg tissue. Our data suggest that DLB alpha-syn seeds are incompletely inactivated by standard autoclave, thus highlighting the need for evaluating laboratory procedures that fully inactivate them.
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