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Brain Sample Preparation After Performing in Utero Electroporation to Proliferating and Differentiated Cells in the Developing Mouse Neocortex

Authors
Shin M.Kosodo Y.
Issue Date
Apr-2024
Publisher
Humana Press Inc.
Citation
Methods in Molecular Biology, v.2794, pp 187 - 200
Pages
14
Journal Title
Methods in Molecular Biology
Volume
2794
Start Page
187
End Page
200
URI
http://scholarworks.bwise.kr/kbri/handle/2023.sw.kbri/1228
DOI
10.1007/978-1-0716-3810-1_16
ISSN
1064-3745
Abstract
In utero electroporation (IUE) enables labeling and manipulating specific types of cells by introducing DNA plasmids with desired promoters. After the surgery, mouse brains are fixed at any stage and analyzed after staining using specific antibodies. Here, we describe the flow of the IUE experiment from the preparation to microscopic observations.
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연구본부 (신경회로 연구그룹)
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