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SCF-Slimb is critical for Glycogen synthase kinase-3 beta-mediated suppression of TAF15-induced neurotoxicity in Drosophila

Authors
Choi, Hyun-JunJoo Cha, SunDo, Hyeon-AhKim, Hyung-JunLee, Jang-WonKim, Kiyoung
Issue Date
Jun-2021
Publisher
Blackwell Publishing Inc.
Keywords
amyotrophic lateral sclerosis; Drosophila; GSK-3 beta; lithium chloride; motor neuron; neurodegeneration; TAF15
Citation
Journal of Neurochemistry, v.157, no.6, pp.2119 - 2127
Journal Title
Journal of Neurochemistry
Volume
157
Number
6
Start Page
2119
End Page
2127
URI
http://scholarworks.bwise.kr/kbri/handle/2023.sw.kbri/319
DOI
10.1111/jnc.15182
ISSN
0022-3042
Abstract
Amyotrophic lateral sclerosis (ALS) is a severe neurodegenerative disorder characterized pathologically by motor neuron degeneration and associated with aggregation of RNA-binding proteins. TATA-binding protein-associated factor 15 (TAF15) accumulates as cytoplasmic aggregates in neuronal cells, and clearance of these aggregates is considered a potential therapeutic strategy for ALS. However, the exact pathogenic mechanism of TAF15-induced neurotoxicity remains to be elucidated. Glycogen synthase kinase-3 (GSK-3) plays a critical role in the protection of ALS pathology. In the present study, we use a transgenic fly model over-expressing human TAF15 to study the protective effects of Shaggy/GSK3 beta on TAF15-induced neuronal toxicity inDrosophilabrain. Transgenic flies were examined for locomotor activity and lithium treatment. The expression level and solubility of TAF15 were assessed with western blotting, whereas immunohistochemistry was used to assess TAF15 aggregation inDrosophilabrain. We have revealed that Shaggy/GSK3 beta was abnormally activated in neurons of TAF15-expressing flies and its inhibition can suppress the defective phenotypes, thereby preventing retinal degeneration and locomotive activity caused by TAF15. We have also found that Shaggy/GSK3 beta inhibition in neuronal cells leads to a reduction in TAF15 levels. Indeed, the F-box proteins Slimb and archipelago genetically interact with TAF15 and control TAF15 protein level inDrosophila. Importantly, SCF(slimb)is a critical regulator for Shaggy/GSK3 beta-mediated suppression of TAF15-induced toxicity inDrosophila. The present study has provided an in vivo evidence supporting the molecular mechanism of GSK3 beta inhibition for protection against TAF15-linked proteinopathies.
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