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Cited 15 time in webofscience Cited 16 time in scopus
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Multiplex Protein Imaging with Secondary Ion Mass Spectrometry Using Metal Oxide Nanoparticle-Conjugated Antibodies

Authors
Moon, Dae WonPark, Young HoLee, Sun YoungLim, HeejinKwak, SuHwaKim, Minseok S.Kim, HyunminKim, EunjooJung, YebinHoe, Hyang-SookKim, SungjeeLim, Dong-KwonKim, Chul-HoonIn, Su-Il
Issue Date
Apr-2020
Publisher
AMER CHEMICAL SOC
Keywords
secondary ion mass spectrometry; multiplex protein imaging; metal oxide nanoparticle; Alzheimer' s disease; protein cluster proximity analysis
Citation
ACS APPLIED MATERIALS & INTERFACES, v.12, no.15, pp.18056 - 18064
Journal Title
ACS APPLIED MATERIALS & INTERFACES
Volume
12
Number
15
Start Page
18056
End Page
18064
URI
http://scholarworks.bwise.kr/kbri/handle/2023.sw.kbri/632
DOI
10.1021/acsami.9b21800
ISSN
1944-8244
Abstract
In spite of recent developments in mass spectrometry imaging techniques, high-resolution multiplex protein bioimaging techniques are required to unveil the complex inter- and intracellular biomolecular interactions for accurate understanding of life phenomena and disease mechanisms. Herein, we report multiplex protein imaging with secondary ion mass spectrometry (SIMS) using metal oxide nanoparticle (MONP)-conjugated antibodies with <300 nm spatial resolution in the low ion dose without ion beam damage because of the high secondary ion yields of the MONPs, which can provide simultaneous imaging of several proteins, especially from cell membranes. We applied our new imaging technique for the study of hippocampal tissue samples from control and Alzheimer's disease (AD) model mice; the proximity of protein clusters in the hippocampus CA1 region showed intriguing dependence on aging and AD progress, suggesting that protein cluster proximity may be helpful for understanding pathological pathways in the microscopic cellular level.
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Hoe, Hyang Sook
연구본부 (퇴행성뇌질환 연구그룹)
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