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Cited 11 time in webofscience Cited 10 time in scopus
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Reliable autapse formation using the single-cell patterning method

Authors
Bang, SeokyoungLee, Byung-JuLee, Seung-RyeolNa, SangcheolJang, Jae MyungKang, MyeongwooKim, Sung-YonMin, Dal-HeeSong, Joon MyongHo, Won-KyungJeon, Noo Li
Issue Date
Jan-2019
Publisher
IOP PUBLISHING LTD
Keywords
single-cell patterning; autapse; multi-electrode arrays
Citation
BIOFABRICATION, v.11, no.1
Journal Title
BIOFABRICATION
Volume
11
Number
1
URI
http://scholarworks.bwise.kr/kbri/handle/2023.sw.kbri/706
DOI
10.1088/1758-5090/aaeb66
ISSN
1758-5082
Abstract
Auto neuronal synapses, or autapses, are aberrant structures where the synaptic contact of a neuron forms onto its own branch. The functions of autapses, however, remain unknown. Here, we introduce a simple patterning method for capturing a single-cell, in which we maintained the isolated cell until it reached maturity, and developed arrays of autapses for electrophysiological analysis using multielectrode arrays (MEA). The pattern arrays were formed by selective patterning of poly-L-lysine and various cell repellent materials. We tested the efficiency of single neuron pattern formed according to materials and pattern dimensions. Autapse formation was verified by immunostaining synaptic markers and physiological measurements via recordings from MEA. The results demonstrated that our multiscale patterning method increased the number of autapses consisting of a single neuron, which matured to connect onto themselves. The proposed patterning method (4.06 +/- 0.33 isolated single-cells mm(-2)) is at least twelve times more efficient and productive than the spray method (0.31 +/- 0.10 isolated single-cells mm(-2)). The spontaneous activity of a single neuron on the patterned MEA occured after 11 d in vitro. The single neuron activity consisted of bursts followed by spike trains (the burst rate was 2.56 min(-1)). This indicates that our method could be used for electrophysiological analysis, including MEA.
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연구본부 (뇌발달질환 연구그룹)
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