Protection of nigral dopaminergic neurons by AAV1 transduction with Rheb(S16H) against neurotoxic inflammation in vivo
- Authors
- Kim, Sehwan; Moon, Gyeong Joon; Oh, Yong-Seok; Park, Jungha; Shin, Won-Ho; Jeong, Jae Yeong; Choi, Kwang Shik; Jin, Byung Kwan; Kholodilov, Nikolai; Burke, Robert E.; Kim, Hyung-Jun; Ha, Chang Man; Lee, Seok-Geun; Kim, Sang Ryong
- Issue Date
- Feb-2018
- Publisher
- NATURE PUBLISHING GROUP
- Citation
- EXPERIMENTAL AND MOLECULAR MEDICINE, v.50
- Journal Title
- EXPERIMENTAL AND MOLECULAR MEDICINE
- Volume
- 50
- URI
- http://scholarworks.bwise.kr/kbri/handle/2023.sw.kbri/755
- DOI
- 10.1038/emm.2017.261
- ISSN
- 1226-3613
- Abstract
- We recently reported that adeno-associated virus serotype 1 (AAV1) transduction of murine nigral dopaminergic (DA) neurons with constitutively active ras homolog enriched in brain with a mutation of serine to histidine at position 16 [Rheb(S16H)] induced the production of neurotrophic factors, resulting in neuroprotective effects on the nigrostriatal DA system in animal models of Parkinson's disease (PD). To further investigate whether AAV1-Rheb(S16H) transduction has neuroprotective potential against neurotoxic inflammation, which is known to be a potential event related to PD pathogenesis, we examined the effects of Rheb(S16H) expression in nigral DA neurons under a neurotoxic inflammatory environment induced by the endogenous microglial activator prothrombin kringle-2 (pKr-2). Our observations showed that Rheb(S16H) transduction played a role in the neuroprotection of the nigrostriatal DA system against pKr-2-induced neurotoxic inflammation, even though there were similar levels of pro-inflammatory cytokines, such as tumor necrosis factor-alpha (TNF-alpha) and interleukin-1-beta (IL-1 beta), in the AAV1-Rheb(S16H)-treated substantia nigra (SN) compared to the SN treated with pKr-2 alone; the neuroprotective effects may be mediated by the activation of neurotrophic signaling pathways following Rheb(S16H) transduction of nigral DA neurons. We conclude that AAV1-Rheb(S16H) transduction of neuronal populations to activate the production of neurotrophic factors and intracellular neurotrophic signaling pathways may offer promise for protecting adult neurons from extracellular neurotoxic inflammation.
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