Detailed Information

Cited 36 time in webofscience Cited 41 time in scopus
Metadata Downloads

Isothiocyanates suppress the invasion and metastasis of tumors by targeting FAK/MMP-9 activity

Authors
Jeong, Yun-JeongCho, Hyun-JiChung, Fung-LungWang, XiantaoHoe, Hyang-SookPark, Kwan-KyuKim, Cheorl-HoChang, Hyeun-WookLee, Sang-RaeChang, Young-Chae
Issue Date
Sep-2017
Publisher
IMPACT JOURNALS LLC
Keywords
isothiocyanates; metastasis; cancer invasion; MMP-9; FAK
Citation
ONCOTARGET, v.8, no.38, pp.63949 - 63962
Journal Title
ONCOTARGET
Volume
8
Number
38
Start Page
63949
End Page
63962
URI
http://scholarworks.bwise.kr/kbri/handle/2023.sw.kbri/820
DOI
10.18632/oncotarget.19213
ISSN
1949-2553
Abstract
Isothiocyanates, which are present as glucosinolate precursors in cruciferous vegetables, have strong activity against various cancers. Here, we compared the anti-metastatic effects of isothiocyanates (benzyl isothiocyanate (BITC), phenethyl isothiocyanate (PEITC), and sulforaphane (SFN)) by examining how they regulate MMP-9 expression. Isothiocyanates, particularly PEITC, suppressed 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced MMP-9 activity and invasion in various cancer cell lines. By contrast, N-methyl phenethylamine, a PEITC analog without an isothiocyanate functional group, had no effect. A reporter gene assay demonstrated that BITC, PEITC, and SFN suppressed TAP-induced MMP-9 expression by inhibiting AP-1 and NF-kappa B in U20S osteosarcoma cells. All three compounds reduced phosphorylation of FAK, ERK1/2, and Akt. In addition, MMP-9 expression was downregulated by inhibiting FAK, ERK1/2, and Akt. Isothiocyanates-mediated inhibition of FAK phosphorylation suppressed phosphorylation of ERK1/2 and Akt in U2OS and A549 cells, along with the translocation of p65 and c-Fos, suggesting that isothiocyanates inhibit MMP-9 expression and cell invasion by blocking phosphorylation of FAK. Furthermore, isothiocyanates, abolished MMP-9 expression and tumor metastasis in vivo with the following efficacy: PEITC>BITC>SFN. Thus, isothiocyanates act as anti-metastatic compounds that suppress MMP-9 activity/expression by inhibiting NF-kappa B and AP-1 via suppression of the FAK/ERK and FAK/Akt signaling pathways.
Files in This Item
There are no files associated with this item.
Appears in
Collections
연구본부 > 퇴행성 뇌질환 연구그룹 > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Hoe, Hyang Sook photo

Hoe, Hyang Sook
연구본부 (퇴행성뇌질환 연구그룹)
Read more

Altmetrics

Total Views & Downloads

BROWSE