Detailed Information

Cited 3 time in webofscience Cited 3 time in scopus
Metadata Downloads

Deneddylase 1 regulates deneddylase activity of the Cop9 signalosome in Drosophila melanogaster

Authors
Kim, KiyoungYoon, JeongsookYim, JeongbinKim, Hyung-Jun
Issue Date
Feb-2017
Publisher
WILEY-BLACKWELL
Keywords
COP9 signalosome; Cullin; DEN1; Drosophila; NEDD8
Citation
INSECT SCIENCE, v.24, no.1, pp.27 - 34
Journal Title
INSECT SCIENCE
Volume
24
Number
1
Start Page
27
End Page
34
URI
http://scholarworks.bwise.kr/kbri/handle/2023.sw.kbri/835
DOI
10.1111/1744-7917.12274
ISSN
1672-9609
Abstract
NEDD8 conjugation of Cullin has an important role in ubiquitin-mediated protein degradation. The COP9 signalosome, of which CSN5 is the major catalytic subunit, is a major Cullin deneddylase. Another deneddylase, Deneddylase 1, has also been shown to process the Nedd8 precursor. In Drosophila, the DEN1 mutants do not have increased levels of Cullin neddylation, but instead show a significant decrease in neddylated Cullin. This characteristic decrease in neddylated Cullins in the DEN1(null) background can be rescued by UAS-dDEN1(WT) overexpression but not by overexpression of mature NEDD8, indicating that this phenotype is distinct from the NEDD8-processing function of DEN1. We examined the role of DEN1-CSN interaction in regulating Cullin neddylation. Overexpression of DEN1 in a CSN5(hypo) background slightly reduced unneddylated Cullin levels. The CSN5, DEN1 double mutation partially rescues the premature lethality associated with the CSN5 single mutation. These results suggest that DEN1 regulates Cullin neddylation by suppressing CSN deneddylase activity.
Files in This Item
There are no files associated with this item.
Appears in
Collections
연구본부 > 치매 연구그룹 > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Kim, Hyung-Jun photo

Kim, Hyung-Jun
연구본부 (치매 연구그룹)
Read more

Altmetrics

Total Views & Downloads

BROWSE