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Cited 141 time in webofscience Cited 167 time in scopus
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ACT-PRESTO: Rapid and consistent tissue clearing and labeling method for 3-dimensional (3D) imaging

Authors
Lee, EunsooChoi, JungyoonJo, YouhwaKim, Joo YeonJang, Yu JinLee, Hye MyeongKim, So YeunLee, Ho-JaeCho, KeunchangJung, NeoncheolHur, Eun MiJeong, Sung JinMoon, CheilChoe, YoungshikRhyu, Im JooKim, HyunSun, Woong
Issue Date
Jan-2016
Publisher
NATURE PORTFOLIO
Citation
SCIENTIFIC REPORTS, v.6
Journal Title
SCIENTIFIC REPORTS
Volume
6
URI
http://scholarworks.bwise.kr/kbri/handle/2023.sw.kbri/867
DOI
10.1038/srep18631
ISSN
2045-2322
Abstract
Understanding the structural organization of organs and organisms at the cellular level is a fundamental challenge in biology. This task has been approached by reconstructing three-dimensional structure from images taken from serially sectioned tissues, which is not only labor-intensive and time-consuming but also error-prone. Recent advances in tissue clearing techniques allow visualization of cellular structures and neural networks inside of unsectioned whole tissues or the entire body. However, currently available protocols require long process times. Here, we present the rapid and highly reproducible ACT-PRESTO (active clarity technique-pressure related efficient and stable transfer of macromolecules into organs) method that clears tissues or the whole body within 1 day while preserving tissue architecture and protein-based signals derived from endogenous fluorescent proteins. Moreover, ACT-PRESTO is compatible with conventional immunolabeling methods and expedites antibody penetration into thick specimens by applying pressure. The speed and consistency of this method will allow high-content mapping and analysis of normal and pathological features in intact organs and bodies.
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연구본부 (뇌발달질환 연구그룹)
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