Human Urine-derived Stem Cells Seeded Surface Modified Composite Scaffold Grafts for Bladder Reconstruction in a Rat Model
- Authors
- Lee Jun Nyung; Chun So Young; Lee Hyo-Jung; Jang Yu-Jin; Choi Seock Hwan; Kim Dae Hwan; Oh Se Heang; Song Phil Hyun; Lee Jin Ho; Kim Jong Kun; Kwon Tae Gyun
- Issue Date
- Dec-2015
- Publisher
- 대한의학회
- Keywords
- Bladder Regeneration; Surface Modified Scaffold; Urine-derived Stem Cells; Basic Fibroblast Growth Factor 2
- Citation
- Journal of Korean Medical Science, v.30, no.12, pp.1754 - 1763
- Journal Title
- Journal of Korean Medical Science
- Volume
- 30
- Number
- 12
- Start Page
- 1754
- End Page
- 1763
- URI
- http://scholarworks.bwise.kr/kbri/handle/2023.sw.kbri/868
- DOI
- 10.3346/jkms.2015.30.12.1754
- ISSN
- 1011-8934
- Abstract
- We conducted this study to investigate the synergistic effect of human urine-derived stem cells (USCs) and surface modified composite scaffold for bladder reconstruction in a rat model. The composite scaffold (Polycaprolactone/Pluronic F127/3 wt% bladder submucosa matrix) was fabricated using an immersion precipitation method, and heparin was immobilized on the surface via covalent conjugation. Basic fibroblast growth factor (bFGF) was loaded onto the heparin-immobilized scaffold by a simple dipping method. In maximal bladder capacity and compliance analysis at 8 weeks post operation, the USCsscaffold(heparin-bFGF) group showed significant functional improvement (2.34 +/- 0.25 mL and 55.09 +/- 11.81 mu L/cm H2O) compared to the other groups (2.60 +/- 0.23 mL and 56.14 +/- 9.00 mu L/cm H2O for the control group, 1.46 +/- 0.18 mL and 34.27 +/- 4.42 mu L/cm H2O for the partial cystectomy group, 1.76 +/- 0.22 mL and 35.62 +/- 6.69 mu L/cm H2O for the scaffold group, and 1.92 +/- 0.29 mL and 40.74 +/- 7.88 mu L/cm H2O for the scaffold(heparin-bFGF) group, respectively). In histological and immunohistochemical analysis, the USC-scaffold(heparin-bFGF) group showed pronounced, well-differentiated, and organized smooth muscle bundle formation, a multi-layered and pan-cytokeratin-positive urothelium, and high condensation of submucosal area. The USCs seeded scaffold(heparin-bFGF) exhibits significantly increased bladder capacity, compliance, regeneration of smooth muscle tissue, multi-layered urothelium, and condensed submucosa layers at the in vivo study.
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