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Human urine-derived stem cells seeded surface modified composite scaffold grafts for bladder reconstruction in a rat model
- Authors
- Lee J.N.; Chun S.Y.; Lee H.-J.; Jang Y.-J.; Choi S.H.; Kim D.H.; Oh S.H.; Song P.H.; Lee J.H.; Kim J.K.; Kwon T.G.
- Issue Date
- Dec-2015
- Publisher
- Korean Academy of Medical Sciencehbear@kams.or.kr
- Keywords
- Bladder Regeneration; Surface Modified Scaffold; Urine-derived Stem Cells; Basic Fibroblast Growth Factor 2
- Citation
- Journal of Korean Medical Science, v.30, no.12, pp.1754 - 1763
- Journal Title
- Journal of Korean Medical Science
- Volume
- 30
- Number
- 12
- Start Page
- 1754
- End Page
- 1763
- ISSN
- 1011-8934
- Abstract
- We conducted this study to investigate the synergistic effect of human urine-derived stem cells (USCs) and surface modified composite scaffold for bladder reconstruction in a rat model. The composite scaffold (Polycaprolactone/Pluronic F127/3 wt% bladder submucosa matrix) was fabricated using an immersion precipitation method, and heparin was immobilized on the surface via covalent conjugation. Basic fibroblast growth factor (bFGF) was loaded onto the heparin-immobilized scaffold by a simple dipping method. In maximal bladder capacity and compliance analysis at 8 weeks post operation, the USCsscaffold
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