Detection of Alpha-Fetoprotein in Hepatocellular Carcinoma Patient Plasma with Graphene Field-Effect Transistor
DC Field | Value | Language |
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dc.contributor.author | Kim, Dae Hoon | - |
dc.contributor.author | Oh, Hong Gi | - |
dc.contributor.author | Park, Woo Hwan | - |
dc.contributor.author | Jeon, Dong Cheol | - |
dc.contributor.author | Lim, Ki Moo | - |
dc.contributor.author | Kim, Hyung Jin | - |
dc.contributor.author | Jang, Byoung Kuk | - |
dc.contributor.author | Song, Kwang Soup | - |
dc.date.available | 2021-04-29T08:42:08Z | - |
dc.date.created | 2020-06-16 | - |
dc.date.issued | 2018-11 | - |
dc.identifier.issn | 1424-8220 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/kumoh/handle/2020.sw.kumoh/19116 | - |
dc.description.abstract | The detection of alpha-fetoprotein (AFP) in plasma is important in the diagnosis of hepatocellular carcinoma (HCC) in humans. We developed a biosensor to detect AFP in HCC patient plasma and in a phosphate buffer saline (PBS) solution using a graphene field-effect transistor (G-FET). The G-FET was functionalized with 1-pyrenebutyric acid N-hydroxysuccinimide ester (PBASE) for immobilization of an anti-AFP antibody. AFP was detected by assessing the shift in the voltage of the Dirac point (Delta V-Dirac) after binding of AFP to the anti-AFP-immobilized G-FET channel surface. This anti-AFP-immobilized G-FET biosensor was able to detect AFP at a concentration of 0.1 ng mL(-1) in PBS, and the detection sensitivity was 16.91 mV. In HCC patient plasma, the biosensor was able to detect AFP at a concentration of 12.9 ng mL(-1), with a detection sensitivity of 5.68 mV. The sensitivity (Delta V-Dirac) depended on the concentration of AFP in either PBS or HCC patient plasma. These data suggest that G-FET biosensors could have practical applications in diagnostics. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.subject | CARBON NANOTUBES | - |
dc.subject | TUMOR-MARKER | - |
dc.subject | BIOSENSOR | - |
dc.subject | FUNCTIONALIZATION | - |
dc.subject | IMMUNOSENSOR | - |
dc.subject | SYSTEM | - |
dc.subject | SHEETS | - |
dc.subject | ACID | - |
dc.title | Detection of Alpha-Fetoprotein in Hepatocellular Carcinoma Patient Plasma with Graphene Field-Effect Transistor | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lim, Ki Moo | - |
dc.contributor.affiliatedAuthor | Song, Kwang Soup | - |
dc.identifier.doi | 10.3390/s18114032 | - |
dc.identifier.wosid | 000451598900442 | - |
dc.identifier.bibliographicCitation | SENSORS, v.18, no.11 | - |
dc.relation.isPartOf | SENSORS | - |
dc.citation.title | SENSORS | - |
dc.citation.volume | 18 | - |
dc.citation.number | 11 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Instruments & Instrumentation | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Engineering, Electrical & Electronic | - |
dc.relation.journalWebOfScienceCategory | Instruments & Instrumentation | - |
dc.subject.keywordPlus | CARBON NANOTUBES | - |
dc.subject.keywordPlus | TUMOR-MARKER | - |
dc.subject.keywordPlus | BIOSENSOR | - |
dc.subject.keywordPlus | FUNCTIONALIZATION | - |
dc.subject.keywordPlus | IMMUNOSENSOR | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | SHEETS | - |
dc.subject.keywordPlus | ACID | - |
dc.subject.keywordAuthor | hepatocellular carcinoma | - |
dc.subject.keywordAuthor | alpha-fetoprotein | - |
dc.subject.keywordAuthor | biosensor | - |
dc.subject.keywordAuthor | graphene | - |
dc.subject.keywordAuthor | field-effect transistor | - |
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