자궁경부암 세포를 위한 광학 모니터링 시스템의 설계 및 분석

Abstract

Flow cytometer is a device for precise analysis of cells by measuring scattering of light and fluorescence according to the cell type. It is used for various purposes in many fields such as molecular biology, immunology, and pathology. However, flow cytometry is expensive and inconvenient in terms of maintenance when simple comparisons between cells are performed or when only simple measurement such as the number of cells is required. In this study, we designed a system that can measure forward scatter, side scatter, and fluorescent scatter using LED and photodiode. As a result, it was possible to measure the fluorescence scattering of cervical cancer cells stained with Calcein AM or DiD, and a high linear relationship with the number of cells was confirmed.